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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ACOT6 Polyclonal antibody

ACOT6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22936-1-AP

Synonyms

ACOT6, acyl CoA thioesterase 6, c14_5530, C14orf42

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using ACOT6 Polyclonal antibody (22936-1-AP)

    WB analysis of mouse liver using 22936-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 22936-1-AP (ACOT6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ACOT6 Polyclonal antibody (22936-1-AP)

    WB analysis of HeLa using 22936-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 22936-1-AP (ACOT6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ACOT6 Polyclonal antibody (22936-1-AP)

    WB analysis of HeLa using 22936-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 22936-1-AP (ACOT6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using ACOT6 Polyclonal antibody (22936-1-AP)

    IHC staining of human kidney using 22936-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney slide using 22936-1-AP (ACOT6 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human kidney tissue using ACOT6 Polyclonal antibody (22936-1-AP)

    IHC staining of human kidney using 22936-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney slide using 22936-1-AP (ACOT6 Antibody) at dilution of 1:50.

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Tested Applications

Positive WB detected inmouse liver tissue, HeLa cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 22936-1-AP is ACOT6 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACOT6 fusion protein Ag18981 相同性解析による交差性が予測される生物種
Full Name acyl-CoA thioesterase 6
Calculated molecular weight 207 aa, 23 kDa
Observed molecular weight 23 kDa
GenBank accession numberBC126380
Gene symbol ACOT6
Gene ID (NCBI) 641372
RRIDAB_11182380
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ACOT6 antibody 22936-1-APDownload protocol
IHC protocol for ACOT6 antibody 22936-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols