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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ACOT9 Monoclonal antibody, PBS Only

ACOT9 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Rat, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3F10G5

Cat no : 66532-1-PBS

Synonyms

ACATE2, ACOT9, Acyl CoA thioester hydrolase 9, acyl CoA thioesterase 9, CGI 16, MT ACT48

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ACOT9 Monoclonal antibody (66532-1-Ig)

    WB analysis using 66532-1-Ig (same clone as 66532-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66532-1-Ig (ACOT9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66532-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW 264.7 cells using ACOT9 Monoclonal antibody (66532-1-Ig)

    WB analysis of RAW 264.7 using 66532-1-Ig (same clone as 66532-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66532-1-Ig (ACOT9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66532-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ACOT9 Monoclonal antibody (66532-1-Ig)

    IHC staining of human colon cancer using 66532-1-Ig (same clone as 66532-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66532-1-Ig (ACOT9 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66532-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ACOT9 Monoclonal antibody (66532-1-Ig)

    IHC staining of human colon cancer using 66532-1-Ig (same clone as 66532-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66532-1-Ig (ACOT9 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66532-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66532-1-PBS targets ACOT9 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Rat, mouse samples.

Tested Reactivity Human, Rat, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen ACOT9 fusion protein Ag8884 相同性解析による交差性が予測される生物種
Full Name acyl-CoA thioesterase 9
Calculated molecular weight 24 kDa, 49 kDa
Observed molecular weight 43-50 kDa
GenBank accession numberBC012573
Gene symbol ACOT9
Gene ID (NCBI) 23597
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ACOT9 (Acyl-coenzyme A thioesterase 9) is a member of the acyl-CoA thioesterase superfamily, which is a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. ACOT9 has 4 isoforms with the molecular mass of 43-50 kDa.