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  • Featured Product
  • KD/KO Validated

ACOX1 Monoclonal antibody, PBS Only

ACOX1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3E12E10

Cat no : 68017-1-PBS

Synonyms

Palmitoyl-CoA oxidase, EC:1.3.3.6, AOX, ACOX 1, ACOX

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ACOX1 Monoclonal antibody (68017-1-Ig)

    WB analysis using 68017-1-Ig (same clone as 68017-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68017-1-Ig (ACOX1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68017-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat liver tissue using ACOX1 Monoclonal antibody (68017-1-Ig)

    WB analysis of rat liver using 68017-1-Ig (same clone as 68017-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 68017-1-Ig (ACOX1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68017-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using ACOX1 Monoclonal antibody (68017-1-Ig)

    WB analysis of HepG2 using 68017-1-Ig (same clone as 68017-1-PBS)

    WB result of ACOX1 antibody (68017-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACOX1 transfected HepG2 cells. This data was developed using the same antibody clone with 68017-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig liver tissue using ACOX1 Monoclonal antibody (68017-1-Ig)

    WB analysis of pig liver using 68017-1-Ig (same clone as 68017-1-PBS)

    pig liver tissue were subjected to SDS PAGE followed by western blot with 68017-1-Ig (ACOX1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68017-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68017-1-PBS targets ACOX1 in WB, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ACOX1 fusion protein Ag28037 相同性解析による交差性が予測される生物種
Full Name acyl-Coenzyme A oxidase 1, palmitoyl
Calculated molecular weight 74 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC010425
Gene symbol ACOX1
Gene ID (NCBI) 51
RRIDAB_2918763
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ACOX1(Peroxisomal acyl-coenzyme A oxidase 1) is the first and rate-limiting enzyme in the peroxisomal fatty acid beta-oxidation pathway and catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. It belongs to the acyl-CoA oxidase family and also named as ACOX, AOX, SCOX. It has 3 isoforms produced by alternative splicing with the molecular mass of 70-74 kDa and can be cleaved post-translationally into a 50-kDa and a 22-kDa subunit between Val468 and Ala469 (PMID: 10672038). Defects in ACOX1 are the cause of adrenoleukodystrophy pseudoneonatal (Pseudo-NALD).