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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ACOX1 Recombinant antibody

ACOX1 Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240754E3

Cat no : 83731-2-RR

Synonyms

Palmitoyl-CoA oxidase, EC:1.3.3.6, AOX, ACOX 1, ACOX

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ACOX1 Recombinant antibody (83731-2-RR)

    WB analysis using 83731-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83731-2-RR (ACOX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovarian cancer using ACOX1 Recombinant antibody (83731-2-RR)

    IHC staining of human ovarian cancer using 83731-2-RR

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83731-2-RR (ACOX1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of A431 cells using ACOX1 Recombinant antibody (83731-2-RR)

    FC experiment of A431 using 83731-2-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug ACOX1 Recombinant antibody (83731-2-RR, Clone:240754E3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83731-2-RR

    Affinity and Kinetic Characterization of 83731-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83731-2-RR against Human ACOX1 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inmouse liver tissue, rat liver tissue
Positive IHC detected inhuman ovarian cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83731-2-RR is ACOX1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ACOX1 fusion protein Ag1405 相同性解析による交差性が予測される生物種
Full Name acyl-Coenzyme A oxidase 1, palmitoyl
Calculated molecular weight 74 kDa
Observed molecular weight70-74 kDa, 22 kDa
GenBank accession numberBC010425
Gene symbol ACOX1
Gene ID (NCBI) 51
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ACOX1(Peroxisomal acyl-coenzyme A oxidase 1) is the first and rate-limiting enzyme in the peroxisomal fatty acid beta-oxidation pathway and catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. It belongs to the acyl-CoA oxidase family and also named as ACOX, AOX, SCOX. It has 3 isoforms produced by alternative splicing with the molecular mass of 70-74 kDa and can be cleaved post-translationally into a 50-kDa and a 22-kDa subunit between Val468 and Ala469 (PMID: 10672038). Defects in ACOX1 are the cause of adrenoleukodystrophy pseudoneonatal (Pseudo-NALD).

Protocols

Product Specific Protocols
WB protocol for ACOX1 antibody 83731-2-RRDownload protocol
IHC protocol for ACOX1 antibody 83731-2-RRDownload protocol
FC protocol for ACOX1 antibody 83731-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols