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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ACOX3 Recombinant antibody

ACOX3 Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241172G8

Cat no : 84027-4-RR

Synonyms

EC:1.3.3.6, BRCOX, BRCACox, Branched-chain acyl-CoA oxidase, ACOX 3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ACOX3 Recombinant antibody (84027-4-RR)

    WB analysis using 84027-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84027-4-RR (ACOX3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of A431 cells using ACOX3 Recombinant antibody (84027-4-RR)

    FC experiment of A431 using 84027-4-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug ACOX3 Recombinant antibody (84027-4-RR, Clone:241172G8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84027-4-RR

    Affinity and Kinetic Characterization of 84027-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84027-4-RR against Human ACOX3 were performed. The affinity constant is 1.05 nM.

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, MCF-7 cells, HL-60 cells, U-251 cells, A549 cells，human placenta tissue
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84027-4-RR is ACOX3 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ACOX3 fusion protein Ag10553 相同性解析による交差性が予測される生物種
Full Name acyl-Coenzyme A oxidase 3, pristanoyl
Calculated molecular weight 624 aa, 70 kDa
Observed molecular weight70-78 kDa
GenBank accession numberBC017053
Gene symbol ACOX3
Gene ID (NCBI) 8310
RRIDAB_3671594
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Acyl-Coenzyme A oxidase 3 also know as pristanoyl -CoA oxidase (ACOX3) is involved in the desaturation of 2-methyl branched fatty acids in peroxisomes.

Protocols

Product Specific Protocols
WB protocol for ACOX3 antibody 84027-4-RRDownload protocol
FC protocol for ACOX3 antibody 84027-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols