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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ACP2 Polyclonal antibody

ACP2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15236-1-AP

Synonyms

LAP, EC:3.1.3.2, ACP 2

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse kidney tissue using ACP2 Polyclonal antibody (15236-1-AP)

    WB analysis of mouse kidney using 15236-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15236-1-AP (ACP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using ACP2 Polyclonal antibody (15236-1-AP)

    WB analysis of mouse kidney using 15236-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15236-1-AP (ACP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using ACP2 Polyclonal antibody (15236-1-AP)

    WB analysis of mouse brain using 15236-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 15236-1-AP (ACP2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using ACP2 Polyclonal antibody (15236-1-AP)

    WB analysis of mouse brain using 15236-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 15236-1-AP (ACP2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using ACP2 Polyclonal antibody (15236-1-AP)

    WB analysis of mouse brain using 15236-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 15236-1-AP (ACP2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using ACP2 Polyclonal antibody (15236-1-AP)

    WB analysis of HepG2 using 15236-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15236-1-AP (ACP2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using ACP2 Polyclonal antibody (15236-1-AP)

    WB analysis of HL-60 using 15236-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 15236-1-AP (ACP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using ACP2 Polyclonal antibody (15236-1-AP)

    WB analysis of HL-60 using 15236-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 15236-1-AP (ACP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using ACP2 Polyclonal antibody (15236-1-AP)

    WB analysis of HepG2 using 15236-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15236-1-AP (ACP2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using ACP2 Polyclonal antibody (15236-1-AP)

    WB analysis of HepG2 using 15236-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15236-1-AP (ACP2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ACP2 Polyclonal antibody (15236-1-AP)

    IF Staining of HepG2 using 15236-1-AP

    Immunofluorescent analysis of HepG2 cells using 15236-1-AP (ACP2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse kidney tissue, HepG2 cells, HL-60 cells, mouse brain tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15236-1-AP targets ACP2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACP2 fusion protein Ag7356 相同性解析による交差性が予測される生物種
Full Name acid phosphatase 2, lysosomal
Calculated molecular weight 48 kDa
Observed molecular weight 63 kDa, 52 kDa
GenBank accession numberBC003160
Gene symbol ACP2
Gene ID (NCBI) 53
RRIDAB_2221939
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ACP2 antibody 15236-1-APDownload protocol
IF protocol for ACP2 antibody 15236-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols