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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ACP6 Polyclonal antibody

ACP6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12965-1-AP

Synonyms

ACP6, ACPL1, LPAP, PACPL1

Validation Data Gallery

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  • Western Blot (WB) analysis of human liver tissue using ACP6 Polyclonal antibody (12965-1-AP)

    WB analysis of human liver using 12965-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 12965-1-AP (ACP6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using ACP6 Polyclonal antibody (12965-1-AP)

    WB analysis of mouse liver using 12965-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 12965-1-AP (ACP6 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ACP6 Polyclonal antibody (12965-1-AP)

    IHC staining of human prostate cancer using 12965-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12965-1-AP (ACP6 antibody) at dilution of 1:100 (under 10x lens).

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Tested Applications

Positive WB detected inhuman liver tissue, mouse liver tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12965-1-AP targets ACP6 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACP6 fusion protein Ag3633 相同性解析による交差性が予測される生物種
Full Name acid phosphatase 6, lysophosphatidic
Calculated molecular weight 428 aa, 49 kDa
Observed molecular weight 49 kDa
GenBank accession numberBC034686
Gene symbol ACP6
Gene ID (NCBI) 51205
RRIDAB_2257842
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ACP6 antibody 12965-1-APDownload protocol
IHC protocol for ACP6 antibody 12965-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols