Validation Data Gallery
Tested Applications
| Positive WB detected in | A2780 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32084-1-AP targets ACP6 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ACP6 fusion protein Ag34812 相同性解析による交差性が予測される生物種 |
| Full Name | acid phosphatase 6, lysophosphatidic |
| Calculated molecular weight | 428 aa, 49 kDa |
| Observed molecular weight | 43 kDa |
| GenBank accession number | BC034686 |
| Gene Symbol | ACP6 |
| Gene ID (NCBI) | 51205 |
| RRID | AB_3670186 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NPH0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Lysophosphatidic acid phosphatase type 6 (ACP6) is an LPA-specific acid phosphatase that hydrolyzes LPA to monoacylglycerol (MAG) and phosphate. Previous studies have shown that ACP6 is localized in the mitochondria with ubiquitous expression throughout all tissues, and high expression levels in kidney, heart, small intestine, muscle, and liver. Through specifi cally hydrolyzing LPA, ACP6 regulates mitochondrial lipid biosynthesis, thereby controlling important mitochondrial functions. It was also shown that ACP6 might be involved in tumor progression, suggesting that ACP6 could be a good candidate marker for the prognosis of esophageal cancer. Uniprot showed that by alternative splicing, ACP6 may have two kinds of shear fragments with molecular weights of 49 and 31kDa respectively. (PMID: 23807634)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ACP6 antibody 32084-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |