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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ACPP Polyclonal antibody

ACPP Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15840-1-AP

Synonyms

acid phosphatase, prostate, ACP 3, ACP3, ACPP, PAP, Prostatic acid phosphatase

Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using ACPP Polyclonal antibody (15840-1-AP)

    WB analysis of PC-3 using 15840-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 15840-1-AP (ACPP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using ACPP Polyclonal antibody (15840-1-AP)

    WB analysis of mouse testis using 15840-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 15840-1-AP (ACPP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using ACPP Polyclonal antibody (15840-1-AP)

    WB analysis of COLO 320 using 15840-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 15840-1-AP (ACPP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using ACPP Polyclonal antibody (15840-1-AP)

    WB analysis of mouse lung using 15840-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 15840-1-AP (ACPP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using ACPP Polyclonal antibody (15840-1-AP)

    WB analysis of MCF-7 using 15840-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 15840-1-AP (ACPP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ACPP Polyclonal antibody (15840-1-AP)

    IHC staining of human prostate cancer using 15840-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15840-1-AP (ACPP antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ACPP Polyclonal antibody (15840-1-AP)

    IHC staining of human prostate cancer using 15840-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15840-1-AP (ACPP antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ACPP Polyclonal antibody (15840-1-AP)

    IHC staining of human prostate cancer using 15840-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15840-1-AP (ACPP antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ACPP Polyclonal antibody (15840-1-AP)

    IHC staining of human prostate cancer using 15840-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15840-1-AP (ACPP antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using ACPP Polyclonal antibody (15840-1-AP)

    IHC staining of human prostate hyperplasia using 15840-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 15840-1-AP (ACPP Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using ACPP Polyclonal antibody (15840-1-AP)

    IHC staining of human prostate hyperplasia using 15840-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 15840-1-AP (ACPP Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using ACPP Polyclonal antibody (15840-1-AP)

    IF Staining of PC-3 using 15840-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 15840-1-AP (ACPP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inPC-3 cells, COLO 320 cells, MCF-7 cells, mouse lung tissue, mouse testis tissue
Positive IHC detected inhuman prostate cancer tissue, human prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15840-1-AP targets ACPP in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitychicken, Rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACPP fusion protein Ag8592 相同性解析による交差性が予測される生物種
Full Name acid phosphatase, prostate
Calculated molecular weight 418 aa, 48 kDa
Observed molecular weight 50-60 kDa
GenBank accession numberBC007460
Gene symbol ACPP
Gene ID (NCBI) 55
RRIDAB_2222099
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ACPP is also named as PAP(prostatic acid phosphatase),5'-nucleotidase, thiamine monophosphatas and Belongs to the histidine acid phosphatase family.This enzyme is a homodimeric glycoprotein with a subunit molecular mass of 50 kDa and is an androgen-stimulated secretory product of the human prostate gland(PMID:8132635).It is also a non-specific phosphatase and has been implicated in the progression of prostate cancer, which inactivates LPA in human seminal plasma(PMID:15280042). This protein has three glycosylation sites(PMID:10639192;12525165).

Protocols

Product Specific Protocols
WB protocol for ACPP antibody 15840-1-APDownload protocol
IHC protocol for ACPP antibody 15840-1-APDownload protocol
IF protocol for ACPP antibody 15840-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
chickenIHC

Biol Reprod

Avian Prostatic Acid Phosphatase: Estrogen Regulation in the Oviduct and Epithelial Cell-Derived Ovarian Carcinomas.

Authors - Hyocheol Bae
View Article
mouseIHC

Am J Pathol

PIK3CG Is a Potential Therapeutic Target in Androgen Receptor-Indifferent Metastatic Prostate Cancer.

Authors - Wen-Cheng Chung
View Article
mouseWB,IF,IP

Eur J Pain

Electroacupuncture relieves neuropathic pain by inhibiting degradation of the ecto-nucleotidase PAP in the dorsal root ganglions of CCI mice.

Authors - Xiaoxin Zhou
View Article
RatIHC, IF

Biochem Biophys Res Commun

Dietary cholesterol affects expression of prostatic acid phosphatase in reproductive organs of male rats.

Authors - Whasun Lim
View Article