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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ACSF2 Recombinant antibody

ACSF2 Recombinant Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241208C3

Cat no : 84033-2-RR

Synonyms

UNQ493/PRO1009, Medium-chain acyl-CoA ligase ACSF2, mitochondrial, EC:6.2.1.2, ACSF 2, 241208C3

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse colon tissue using ACSF2 Recombinant antibody (84033-2-RR)

    IHC staining of mouse colon using 84033-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84033-2-RR (ACSF2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using ACSF2 Recombinant antibody (84033-2-RR)

    IF Staining of A549 using 84033-2-RR

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using ACSF2 antibody (84033-2-RR, Clone: 241208C3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using ACSF2 Recombinant antibody (84033-2-RR)

    IF Staining of A549 using 84033-2-RR

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using ACSF2 antibody (84033-2-RR, Clone: 241208C3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84033-2-RR targets ACSF2 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ACSF2 fusion protein Ag9199 相同性解析による交差性が予測される生物種
Full Name acyl-CoA synthetase family member 2
Calculated molecular weight 615 aa, 68 kDa
GenBank accession numberBC012053
Gene symbol ACSF2
Gene ID (NCBI) 80221
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for ACSF2 antibody 84033-2-RRDownload protocol
IF protocol for ACSF2 antibody 84033-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols