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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

ACSF3 Polyclonal antibody

ACSF3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25484-1-AP

Synonyms

PSEC0197, Malonate--CoA ligase ACSF3, mitochondrial, EC:6.2.1.76, Acyl-CoA synthetase family member 3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ACSF3 Polyclonal antibody (25484-1-AP)

    WB analysis using 25484-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25484-1-AP (ACSF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using ACSF3 Polyclonal antibody (25484-1-AP)

    IHC staining of human stomach using 25484-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 25484-1-AP (ACSF3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse brain tissue, mouse heart tissue, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25484-1-AP targets ACSF3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACSF3 fusion protein Ag21979 相同性解析による交差性が予測される生物種
Full Name acyl-CoA synthetase family member 3
Calculated molecular weight 576 aa, 64 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC028399
Gene symbol ACSF3
Gene ID (NCBI) 197322
RRIDAB_2880099
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ACSF3 antibody 25484-1-APDownload protocol
IHC protocol for ACSF3 antibody 25484-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Bioconjug Chem

Genetically-encoded FapR-NLuc as a biosensor to determine malonyl-CoA in situ at subcellular scales.

Authors - Yipeng Du
  • KD Validated
View Article
mouseWB

Sci Transl Med

Aberrant methylmalonylation underlies methylmalonic acidemia and is attenuated by an engineered sirtuin

Authors - PamelaSara E Head
View Article