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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ACSL3 Polyclonal antibody

ACSL3 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 30214-1-AP

Synonyms

ACS3, ACSL3, FACL3, LACS 3, LACS3, PRO2194

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ACSL3 Polyclonal antibody (30214-1-AP)

    WB analysis using 30214-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30214-1-AP (ACSL3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ACSL3 Polyclonal antibody (30214-1-AP)

    WB analysis using 30214-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30214-1-AP (ACSL3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of LNCaP cells using ACSL3 Polyclonal antibody (30214-1-AP)

    IP experiment of LNCaP using 30214-1-AP

    IP result of anti-ACSL3 (IP:30214-1-AP, 4ug; Detection:30214-1-AP 1:4000) with LNCaP cells lysate 1360 ug.

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Tested Applications

Positive WB detected inDU 145 cells, HuH-7 cells, LNCaP cells, MKN-45 cells, mouse kidney tissue
Positive IP detected inLNCaP cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30214-1-AP targets ACSL3 in WB, IP, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACSL3 fusion protein Ag33005 相同性解析による交差性が予測される生物種
Full Name acyl-CoA synthetase long-chain family member 3
Calculated molecular weight 80 kDa
Observed molecular weight72 kDa
GenBank accession numberBC041692
Gene symbol ACSL3
Gene ID (NCBI) 2181
RRIDAB_2935532
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ACSL3, also named as ACS3, FACL3 and LACS3, belongs to the ATP-dependent AMP-binding enzyme family. Acyl-CoA synthetases (ACSL) activate long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. ACSL3 mediates hepatic lipogenesis. Preferentially uses myristate, laurate, arachidonate and eicosapentaenoate as substrates. Has mainly an anabolic role in energy metabolism. Required for the incorporation of fatty acids into phosphatidylcholine, the major phospholipid located on the surface of VLDL (very low density lipoproteins). The antibody is specific to ACSL3.

Protocols

Product Specific Protocols
WB protocol for ACSL3 antibody 30214-1-APDownload protocol
IP protocol for ACSL3 antibody 30214-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols