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KD/KO Validated

ACSL4/FACL4 Monoclonal antibody

Name: ACSL4/FACL4 Monoclonal antibody
Brand: Proteintech
SKU: 66617-1-Ig

ACSL4/FACL4 Monoclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human and More (1)

Applications

WB, IHC, IF, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

CloneNo.

1H5D3

Review Product

Cat no : 66617-1-Ig

Synonyms

ACSL4, FACL4, EC:6.2.1.15, Arachidonate--CoA ligase, ACSL 4

Validation Data Gallery

Filter:

AllWBIHCFC (Intra)

WB analysis using 66617-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 66617-1-Ig (ACSL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 66617-1-Ig
WB result of ACSL4 antibody (66617-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACSL4 transfected HepG2 cells.
WB analysis of HeLa using 66617-1-Ig
HeLa cells were subjected to SDS PAGE followed by western blot with 66617-1-Ig (ACSL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 66617-1-Ig
HepG2 cells were subjected to SDS PAGE followed by western blot with 66617-1-Ig (ACSL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of Caco-2 using 66617-1-Ig
Caco-2 cells were subjected to SDS PAGE followed by western blot with 66617-1-Ig (ACSL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 66617-1-Ig
COLO 320 cells were subjected to SDS PAGE followed by western blot with 66617-1-Ig (ACSL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 66617-1-Ig
L02 cells were subjected to SDS PAGE followed by western blot with 66617-1-Ig (ACSL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IHC staining of human cerebellum using 66617-1-Ig
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66617-1-Ig (ACSL4 antibody) at dilution of 1:500 (under 10x lens).
IHC staining of human cerebellum using 66617-1-Ig
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66617-1-Ig (ACSL4 antibody) at dilution of 1:500 (under 40x lens).
FC experiment of HEK-293T using 66617-1-Ig
1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human ACSL4 (66617-1-Ig, Clone:1H5D3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 66617-1-Ig

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, HepG2 cells, Caco-2 cells, COLO 320 cells, L02 cells, HT-29 cells
Positive IHC detected in	human cerebellum tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive FC (Intra) detected in	HEK-293T cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:3000-1:10000
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 26 publications below
IHC	See 1 publications below
IF	See 1 publications below

Product Information

66617-1-Ig targets ACSL4/FACL4 in WB, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, rat
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	ACSL4/FACL4 fusion protein Ag18085 相同性解析による交差性が予測される生物種
Full Name	acyl-CoA synthetase long-chain family member 4
Calculated molecular weight	711 aa, 79 kDa
Observed molecular weight	74 kDa
GenBank accession number	BC034959
Gene symbol	ACSL4
Gene ID (NCBI)	2182
RRID	AB_2881977
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for ACSL4/FACL4 antibody 66617-1-Ig	Download protocol
IHC protocol for ACSL4/FACL4 antibody 66617-1-Ig	Download protocol
FC protocol for ACSL4/FACL4 antibody 66617-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Death Dis RAB17 promotes endometrial cancer progression by inhibiting TFRC-dependent ferroptosis Authors - Xing Zhou View Article
	WB,IF	Phytomedicine MaiJiTong granule attenuates atherosclerosis by reducing ferroptosis via activating STAT6-mediated inhibition of DMT1 and SOCS1/p53 pathways in LDLR-/- mice Authors - Jia Shi View Article
human	WB	J Cell Biol The deubiquitinase ZRANB1 is an E3 ubiquitin ligase for SLC7A11 and regulates ferroptotic resistance Authors - Shan Huang View Article
human	WB	Free Radic Biol Med Targeting the Na+/K+ ATPase DR-region with DR-Ab improves doxorubicin-induced cardiotoxicity Authors - Bin Leng View Article
rat	WB	PLoS Pathog KSHV vIL-6 promotes SIRT3-induced deacetylation of SERBP1 to inhibit ferroptosis and enhance cellular transformation by inducing lipoyltransferase 2 mRNA degradation Authors - Jing Zhou View Article
human	WB	J Med Chem Seco-Lupane Triterpene Derivatives Induce Ferroptosis through GPX4/ACSL4 Axis and Target Cyclin D1 to Block the Cell Cycle. Authors - Haohao Wang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ACSL4/FACL4 Monoclonal antibody

ACSL4/FACL4 Monoclonal Antibody for WB, IHC, FC (Intra), ELISA

Validation Data Gallery

WB analysis using 66617-1-Ig

WB analysis of HepG2 using 66617-1-Ig

WB analysis of HeLa using 66617-1-Ig

WB analysis of HepG2 using 66617-1-Ig

WB analysis of Caco-2 using 66617-1-Ig

WB analysis of COLO 320 using 66617-1-Ig

WB analysis of L02 using 66617-1-Ig

IHC staining of human cerebellum using 66617-1-Ig

IHC staining of human cerebellum using 66617-1-Ig

FC experiment of HEK-293T using 66617-1-Ig

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

RAB17 promotes endometrial cancer progression by inhibiting TFRC-dependent ferroptosis

MaiJiTong granule attenuates atherosclerosis by reducing ferroptosis via activating STAT6-mediated inhibition of DMT1 and SOCS1/p53 pathways in LDLR-/- mice

The deubiquitinase ZRANB1 is an E3 ubiquitin ligase for SLC7A11 and regulates ferroptotic resistance

Targeting the Na+/K+ ATPase DR-region with DR-Ab improves doxorubicin-induced cardiotoxicity

KSHV vIL-6 promotes SIRT3-induced deacetylation of SERBP1 to inhibit ferroptosis and enhance cellular transformation by inducing lipoyltransferase 2 mRNA degradation

Seco-Lupane Triterpene Derivatives Induce Ferroptosis through GPX4/ACSL4 Axis and Target Cyclin D1 to Block the Cell Cycle.