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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ACSL4/FACL4 Recombinant antibody (81196-1-RR)

    WB analysis using 81196-1-RR (same clone as 81196-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81196-1-RR (ACSL4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81196-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using ACSL4/FACL4 Recombinant antibody (81196-1-RR)

    WB analysis of HepG2 using 81196-1-RR (same clone as 81196-1-PBS)

    WB result of ACSL4 antibody (81196-1-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACSL4 transfected HepG2 cells. This data was developed using the same antibody clone with 81196-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using ACSL4/FACL4 Recombinant antibody (81196-1-RR)

    IP experiment of HeLa using 81196-1-RR (same clone as 81196-1-PBS)

    IP result of anti-ACSL4/FACL4 (IP:81196-1-RR, 4ug; Detection:81196-1-RR 1:3000) with HeLa cells lysate 1560 ug. This data was developed using the same antibody clone with 81196-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using ACSL4/FACL4 Recombinant antibody (81196-1-RR)

    IHC staining of human stomach cancer using 81196-1-RR (same clone as 81196-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 81196-1-RR (ACSL4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81196-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ACSL4/FACL4 Recombinant antibody (81196-1-RR)

    IHC staining of human liver cancer using 81196-1-RR (same clone as 81196-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81196-1-RR (ACSL4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81196-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using ACSL4/FACL4 Recombinant antibody (81196-1-RR)

    IHC staining of human stomach cancer using 81196-1-RR (same clone as 81196-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 81196-1-RR (ACSL4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81196-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ACSL4/FACL4 Recombinant antibody (81196-1-RR)

    IHC staining of human liver cancer using 81196-1-RR (same clone as 81196-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81196-1-RR (ACSL4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81196-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using ACSL4/FACL4 Recombinant antibody (81196-1-RR)

    IHC staining of rat brain using 81196-1-RR (same clone as 81196-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 81196-1-RR (ACSL4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81196-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using ACSL4/FACL4 Recombinant antibody (81196-1-RR)

    IHC staining of rat brain using 81196-1-RR (same clone as 81196-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 81196-1-RR (ACSL4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81196-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

ACSL4/FACL4 Recombinant antibody, PBS Only

ACSL4/FACL4 Uni-rAbTM Recombinant Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 81196-1-PBS
Clone No.3I9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

ACSL4, FACL4, EC:6.2.1.15, Arachidonate--CoA ligase, ACSL 4

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81196-1-PBS targets ACSL4/FACL4 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ACSL4/FACL4 fusion protein Ag18085 相同性解析による交差性が予測される生物種
Full Name acyl-CoA synthetase long-chain family member 4
Calculated molecular weight 711 aa, 79 kDa
Observed molecular weight70 kDa, 75 kDa
GenBank accession numberBC034959
Gene Symbol ACSL4
Gene ID (NCBI) 2182
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO60488
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Acyl-CoA synthetase long-chain family member 4 (Acsl4), an important enzyme involved in lipid metabolism, participates in ferroptosis by converting free AA into arachidonoyl-CoA to generate lipid hydroperoxides. Recent studies revealed that ACSL4 is involved in biological responses including inflammation, steroidogenesis, cell death, female fertility, and cancer. Acsl4 has two isoforms: 79 kDa and 75 kDa. ACSL4 also can be detected in 70 kDa(PMID:35326233).