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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ACSL6 Polyclonal antibody (13847-1-AP)

    WB analysis using 13847-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13847-1-AP (ACSL6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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ACSL6 Polyclonal antibody

ACSL6 Polyclonal Antibody for WB, ELISA
Cat No. 13847-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

LACS 6, FACL6, EC:6.2.1.3, EC:6.2.1.15, Arachidonate--CoA ligase

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration:  150 μL, 550 μg/ml
20 μL, 550 μg/ml
150 μL, 550 μg/ml

¥67,000/ 150 μL

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Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13847-1-AP targets ACSL6 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACSL6 fusion protein Ag4817 相同性解析による交差性が予測される生物種
Full Name acyl-CoA synthetase long-chain family member 6
Calculated molecular weight 78 kDa
Observed molecular weight74 kDa
GenBank accession numberBC047453
Gene Symbol ACSL6
Gene ID (NCBI) 23305
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UKU0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ACSL6 (Acyl-CoA Synthetase Long Chain Family Member 6) is a long-chain lipoyl-coenzyme A synthetase that is mainly responsible for catalyzing the binding of long-chain fatty acids to coenzyme A to form acyl-coenzyme A (Acyl-CoA). ACSL6 is involved in fatty acid metabolic transporter and the synthesis of cell membrane phospholipids, which affects the cell structure and function. ACSL6 plays an extremely important role in the pathogenesis of cancers such as hepatocellular carcinoma, lung cancer and neuroblastoma, suggesting that it may serve as a biomarker and therapeutic target for cancer therapy.

Protocols

Product Specific Protocols
WB protocol for ACSL6 antibody 13847-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols