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Target

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Species Reactivity

Species Reactivity

Conjugate

Conjugate

ACSM5 Polyclonal antibody

ACSM5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16591-1-AP

Synonyms

MACS3, EC:6.2.1.2, Acyl-coenzyme A synthetase ACSM5, mitochondrial, Acyl-CoA synthetase medium-chain family member 5, ACSM 5

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using ACSM5 Polyclonal antibody (16591-1-AP)

    WB analysis of mouse liver using 16591-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 16591-1-AP (ACSM5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ACSM5 Polyclonal antibody (16591-1-AP)

    IHC staining of human liver cancer using 16591-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16591-1-AP (ACSM5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ACSM5 Polyclonal antibody (16591-1-AP)

    IHC staining of human liver cancer using 16591-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16591-1-AP (ACSM5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using ACSM5 Polyclonal antibody (16591-1-AP)

    IHC staining of mouse liver using 16591-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16591-1-AP (ACSM5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ACSM5 Polyclonal antibody (16591-1-AP)

    IHC staining of human liver cancer using 16591-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16591-1-AP (ACSM5 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ACSM5 Polyclonal antibody (16591-1-AP)

    IHC staining of human liver cancer using 16591-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16591-1-AP (ACSM5 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using ACSM5 Polyclonal antibody (16591-1-AP)

    IHC staining of mouse liver using 16591-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16591-1-AP (ACSM5 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using ACSM5 Polyclonal antibody (16591-1-AP)

    IHC staining of mouse liver using 16591-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16591-1-AP (ACSM5 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inmouse liver tissue
Positive IHC detected inhuman liver cancer tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

16591-1-AP targets ACSM5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACSM5 fusion protein Ag9373 相同性解析による交差性が予測される生物種
Full Name acyl-CoA synthetase medium-chain family member 5
Calculated molecular weight 208 aa, 23 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC016703
Gene symbol ACSM5
Gene ID (NCBI) 54988
RRIDAB_2878284
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ACSM5(Acyl-CoA synthetase medium-chain family member 5) is also named as MACS3 and belongs to the ATP-dependent AMP-binding enzyme family. ACSM5 has medium-chain fatty acid:CoA ligase activity with broad substrate specificity (in vitro). This protein has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for ACSM5 antibody 16591-1-APDownload protocol
IHC protocol for ACSM5 antibody 16591-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Metab

Calponin 2 harnesses metabolic reprogramming to determine kidney fibrosis

Authors - Yuan Gui
View Article