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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ACSM5 Monoclonal antibody

ACSM5 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2D5D6

Cat no : 67334-1-Ig

Synonyms

ACSM5, FLJ20581, MACS3

Validation Data Gallery

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  • Western Blot (WB) analysis of 4T1 cells using ACSM5 Monoclonal antibody (67334-1-Ig)

    WB analysis of 4T1 using 67334-1-Ig

    4T1 cells were subjected to SDS PAGE followed by western blot with 67334-1-Ig (ACSM5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat liver tissue using ACSM5 Monoclonal antibody (67334-1-Ig)

    WB analysis of rat liver using 67334-1-Ig

    rat liver tissue were subjected to SDS PAGE followed by western blot with 67334-1-Ig (ACSM5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of 4T1 cells using ACSM5 Monoclonal antibody (67334-1-Ig)

    WB analysis of 4T1 using 67334-1-Ig

    4T1 cells were subjected to SDS PAGE followed by western blot with 67334-1-Ig (ACSM5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MDA-MB-231 cells using ACSM5 Monoclonal antibody (67334-1-Ig)

    WB analysis of MDA-MB-231 using 67334-1-Ig

    MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with 67334-1-Ig (ACSM5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ACSM5 Monoclonal antibody (67334-1-Ig)

    IHC staining of human liver cancer using 67334-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67334-1-Ig (ACSM5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ACSM5 Monoclonal antibody (67334-1-Ig)

    IHC staining of human liver cancer using 67334-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67334-1-Ig (ACSM5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ACSM5 Monoclonal antibody (67334-1-Ig)

    IHC staining of human liver cancer using 67334-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67334-1-Ig (ACSM5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ACSM5 Monoclonal antibody (67334-1-Ig)

    IHC staining of human liver cancer using 67334-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67334-1-Ig (ACSM5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ACSM5 Monoclonal antibody (67334-1-Ig)

    IF Staining of HepG2 using 67334-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ACSM5 antibody (67334-1-Ig, Clone: 2D5D6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

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Tested Applications

Positive WB detected in4T1 cells, MDA-MB-231 cells, rat liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

67334-1-Ig targets ACSM5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ACSM5 fusion protein Ag10056 相同性解析による交差性が予測される生物種
Full Name acyl-CoA synthetase medium-chain family member 5
Calculated molecular weight 208 aa, 23 kDa
Observed molecular weight 60-65 kDa
GenBank accession numberBC016703
Gene symbol ACSM5
Gene ID (NCBI) 54988
RRIDAB_2882592
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ACSM5(Acyl-CoA synthetase medium-chain family member 5) is also named as MACS3 and belongs to the ATP-dependent AMP-binding enzyme family. ACSM5 has medium-chain fatty acid:CoA ligase activity with broad substrate specificity (in vitro). This protein has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for ACSM5 antibody 67334-1-IgDownload protocol
IHC protocol for ACSM5 antibody 67334-1-IgDownload protocol
IF protocol for ACSM5 antibody 67334-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Arthritis Res Ther

Integrative analysis of genome-wide DNA methylation and single-nucleotide polymorphism identified ACSM5 as a suppressor of lumbar ligamentum flavum hypertrophy.

Authors - Yanlin Cao
View Article