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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ACSM5 Recombinant antibody

ACSM5 Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241400E7

Cat no : 84444-1-RR

Synonyms

MACS3, EC:6.2.1.2, Acyl-coenzyme A synthetase ACSM5, mitochondrial, Acyl-CoA synthetase medium-chain family member 5, ACSM 5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ACSM5 Recombinant antibody (84444-1-RR)

    WB analysis using 84444-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84444-1-RR (ACSM5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HepG2 cells using ACSM5 Recombinant antibody (84444-1-RR)

    FC experiment of HepG2 using 84444-1-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug ACSM5 Recombinant antibody (84444-1-RR, Clone:241400E7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84444-1-RR

    Affinity and Kinetic Characterization of 84444-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84444-1-RR against Human ACSM5 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inmouse liver tissue, rat liver tissue, human kidney tissue
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84444-1-RR is ACSM5 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ACSM5 fusion protein Ag9373 相同性解析による交差性が予測される生物種
Full Name acyl-CoA synthetase medium-chain family member 5
Calculated molecular weight 208 aa, 23 kDa
Observed molecular weight 60-65 kDa
GenBank accession numberBC016703
Gene symbol ACSM5
Gene ID (NCBI) 54988
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ACSM5(Acyl-CoA synthetase medium-chain family member 5) is also named as MACS3 and belongs to the ATP-dependent AMP-binding enzyme family. ACSM5 has medium-chain fatty acid:CoA ligase activity with broad substrate specificity (in vitro). This protein has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for ACSM5 antibody 84444-1-RRDownload protocol
FC protocol for ACSM5 antibody 84444-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols