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  • Featured Product
  • KD/KO Validated

ACSS1 Polyclonal antibody, PBS Only

ACSS1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 17138-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ACSS1 Polyclonal antibody (17138-1-AP)

    WB analysis using 17138-1-AP (same clone as 17138-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17138-1-AP (ACSS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17138-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse kidney tissue using ACSS1 Polyclonal antibody (17138-1-AP)

    WB analysis of mouse kidney using 17138-1-AP (same clone as 17138-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17138-1-AP (ACSS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17138-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse kidney tissue using ACSS1 Polyclonal antibody (17138-1-AP)

    WB analysis of mouse kidney using 17138-1-AP (same clone as 17138-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17138-1-AP (ACSS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17138-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ACSS1 Polyclonal antibody (17138-1-AP)

    WB analysis using 17138-1-AP (same clone as 17138-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17138-1-AP (ACSS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17138-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Caco-2 cells using ACSS1 Polyclonal antibody (17138-1-AP)

    WB analysis of Caco-2 using 17138-1-AP (same clone as 17138-1-PBS)

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 17138-1-AP (ACSS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17138-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Caco-2 cells using ACSS1 Polyclonal antibody (17138-1-AP)

    WB analysis of Caco-2 using 17138-1-AP (same clone as 17138-1-PBS)

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 17138-1-AP (ACSS1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17138-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse kidney tissue using ACSS1 Polyclonal antibody (17138-1-AP)

    IP experiment of mouse kidney using 17138-1-AP (same clone as 17138-1-PBS)

    IP result of anti-ACSS1 (IP:17138-1-AP, 4ug; Detection:17138-1-AP 1:700) with mouse kidney tissue lysate 4000ug. This data was developed using the same antibody clone with 17138-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ACSS1 Polyclonal antibody (17138-1-AP)

    IHC staining of human liver cancer using 17138-1-AP (same clone as 17138-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17138-1-AP (ACSS1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 17138-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ACSS1 Polyclonal antibody (17138-1-AP)

    IHC staining of human liver cancer using 17138-1-AP (same clone as 17138-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17138-1-AP (ACSS1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 17138-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17138-1-PBS targets ACSS1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACSS1 fusion protein Ag10896 相同性解析による交差性が予測される生物種
Full Name acyl-CoA synthetase short-chain family member 1
Calculated molecular weight 689 aa, 75 kDa
Observed molecular weight 70-75 kDa
GenBank accession numberBC039261
Gene symbol ACSS1
Gene ID (NCBI) 84532
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The ACSS (acetyl-CoA synthetase) enzyme is the sole known mammalian enzyme that can catalyze the conversion of free acetate into acetyl coenzyme A (acetyl-CoA). The three known isoforms of human ACSS are termed ACSS1, ACSS2, and ACSS3. The main substrate of ACSS1 and ACSS2 is acetate, while the preferential substrate of ACSS3 is propionate. Two acetate related enzymes, ACSS1(GeneID: 84532) and ACSS2 (GeneID:55902) difer in their tissue distribution and subcellular localization. On the one hand, as a mitochondrial matrix enzyme, ACSS1 is expressed mainly in cardiac and skeletal muscle as well as brown adipose tissue. On the other hand, as a nuclear and cytoplasmic enzyme, ACSS2 is strongly expressed in the liver, kidney and heart and moderately expressed in the brain and testis.ACSS2 participates in lipid synthesis and facilitates protein acetylation by generating acetyl-CoA, while ACSS1 is involved in acetate oxidation. The functional diferences in these enzymes involve energy production through the tricarboxylic acid (TCA) cycle. Due to its more thorough utilization of intracellular acetate, ACSS2 is expressed in almost all cell types under diferent physiological conditions.