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  • Featured Product
  • KD/KO Validated

ACSS2 Polyclonal antibody, PBS Only

ACSS2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 16087-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ACSS2 Polyclonal antibody (16087-1-AP)

    WB analysis using 16087-1-AP (same clone as 16087-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16087-1-AP (ACSS2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16087-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ACSS2 Polyclonal antibody (16087-1-AP)

    WB analysis using 16087-1-AP (same clone as 16087-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16087-1-AP (ACSS2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16087-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using ACSS2 Polyclonal antibody (16087-1-AP)

    WB analysis of mouse liver using 16087-1-AP (same clone as 16087-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 16087-1-AP (ACSS2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16087-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using ACSS2 Polyclonal antibody (16087-1-AP)

    IP experiment of HepG2 using 16087-1-AP (same clone as 16087-1-PBS)

    IP result of anti-ACSS2 (IP:16087-1-AP, 4ug; Detection:16087-1-AP 1:500) with HepG2 cells lysate 5600 ug. This data was developed using the same antibody clone with 16087-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using ACSS2 Polyclonal antibody (16087-1-AP)

    IHC staining of human brain using 16087-1-AP (same clone as 16087-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 16087-1-AP (ACSS2 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16087-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using ACSS2 Polyclonal antibody (16087-1-AP)

    IHC staining of human brain using 16087-1-AP (same clone as 16087-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 16087-1-AP (ACSS2 synthetase antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16087-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16087-1-PBS targets ACSS2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACSS2 fusion protein Ag9121 相同性解析による交差性が予測される生物種
Full Name acyl-CoA synthetase short-chain family member 2
Calculated molecular weight 701 aa, 79 kDa
Observed molecular weight 79 kDa
GenBank accession numberBC010141
Gene symbol ACSS2
Gene ID (NCBI) 55902
RRIDAB_2878216
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Acetyl-CoA Synthetase 2 (ACSS2) is also named as acyl-CoA synthetase short-chain family member 2. Acetyl-CoA Synthetase 2 (ACSS2) is responsible for lipogenesis by synthesizing acetyl-CoA from acetate for lipogenesis and promotes acetylation in nuclear (PMID: 29444517). It is also required for cell migration and invasion in renal cell carcinoma (RCC) through the promotion of autophagy (PMID: 29281714). ACSS2 has two isoforms with the molecular weight of 78 and 80 kDa.