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  • Featured Product
  • KD/KO Validated

ACSS2 Polyclonal antibody, PBS Only

ACSS2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 16087-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ACSS2 Polyclonal antibody (16087-1-AP)

    WB analysis using 16087-1-AP (same clone as 16087-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16087-1-AP (ACSS2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16087-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ACSS2 Polyclonal antibody (16087-1-AP)

    WB analysis using 16087-1-AP (same clone as 16087-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16087-1-AP (ACSS2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16087-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using ACSS2 Polyclonal antibody (16087-1-AP)

    WB analysis of mouse liver using 16087-1-AP (same clone as 16087-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 16087-1-AP (ACSS2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16087-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using ACSS2 Polyclonal antibody (16087-1-AP)

    IP experiment of HepG2 using 16087-1-AP (same clone as 16087-1-PBS)

    IP result of anti-ACSS2 (IP:16087-1-AP, 4ug; Detection:16087-1-AP 1:500) with HepG2 cells lysate 5600 ug. This data was developed using the same antibody clone with 16087-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using ACSS2 Polyclonal antibody (16087-1-AP)

    IHC staining of human brain using 16087-1-AP (same clone as 16087-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 16087-1-AP (ACSS2 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16087-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using ACSS2 Polyclonal antibody (16087-1-AP)

    IHC staining of human brain using 16087-1-AP (same clone as 16087-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 16087-1-AP (ACSS2 synthetase antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16087-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16087-1-PBS targets ACSS2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACSS2 fusion protein Ag9121 相同性解析による交差性が予測される生物種
Full Name acyl-CoA synthetase short-chain family member 2
Calculated molecular weight 701 aa, 79 kDa
Observed molecular weight 79 kDa
GenBank accession numberBC010141
Gene symbol ACSS2
Gene ID (NCBI) 55902
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Acetyl-CoA Synthetase 2 (ACSS2) is also named as acyl-CoA synthetase short-chain family member 2. Acetyl-CoA Synthetase 2 (ACSS2) is responsible for lipogenesis by synthesizing acetyl-CoA from acetate for lipogenesis and promotes acetylation in nuclear (PMID: 29444517). It is also required for cell migration and invasion in renal cell carcinoma (RCC) through the promotion of autophagy (PMID: 29281714). ACSS2 has two isoforms with the molecular weight of 78 and 80 kDa.