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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ACSS2 Recombinant antibody (84774-7-RR)

    WB analysis using 84774-7-RR (same clone as 84774-7-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84774-7-RR (ACSS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84774-7-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Caco-2 cells using ACSS2 Recombinant antibody (84774-7-RR)

    FC experiment of Caco-2 using 84774-7-RR (same clone as 84774-7-PBS)

    1x10^6 Caco-2 cells were intracellularly stained with 0.25 ug ACSS2 Recombinant antibody (84774-7-RR, Clone:242253C6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84774-7-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using ACSS2 Recombinant antibody (84774-7-RR)

    FC experiment of HepG2 using 84774-7-RR (same clone as 84774-7-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug ACSS2 Recombinant antibody (84774-7-RR, Clone:242253C6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84774-7-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84774-7-RR

    Affinity and Kinetic Characterization of 84774-7-RR

    Biolayer interferometry (BLl) kinetic assays of 84774-7-RR against Human ACSS2 were performed. The affinity constant is 91.2 nM.

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ACSS2 Recombinant antibody, PBS Only

ACSS2 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 84774-7-PBS
Clone No.242253C6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

Acetyl-CoA synthetase 1, Acetyl-CoA synthetase, Acetate--CoA ligase, AceCS1, ACAS2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84774-7-PBS targets ACSS2 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ACSS2 fusion protein Ag9121 相同性解析による交差性が予測される生物種
Full Name acyl-CoA synthetase short-chain family member 2
Calculated molecular weight 701 aa, 79 kDa
Observed molecular weight 79 kDa
GenBank accession numberBC010141
Gene Symbol ACSS2
Gene ID (NCBI) 55902
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9NR19
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Acetyl-CoA Synthetase 2 (ACSS2) is also named as acyl-CoA synthetase short-chain family member 2. Acetyl-CoA Synthetase 2 (ACSS2) is responsible for lipogenesis by synthesizing acetyl-CoA from acetate for lipogenesis and promotes acetylation in nuclear (PMID: 29444517). It is also required for cell migration and invasion in renal cell carcinoma (RCC) through the promotion of autophagy (PMID: 29281714).