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Validation Data Gallery

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  • Western Blot (WB) analysis of human skeletal muscle tissue using ACTA1-Specific Polyclonal antibody (17521-1-AP)

    WB analysis of human skeletal muscle using 17521-1-AP (same clone as 17521-1-PBS)

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17521-1-AP (ACTA1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17521-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using ACTA1-Specific Polyclonal antibody (17521-1-AP)

    WB analysis of mouse skeletal muscle using 17521-1-AP (same clone as 17521-1-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17521-1-AP (ACTA1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17521-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat skeletal muscle tissue using ACTA1-Specific Polyclonal antibody (17521-1-AP)

    WB analysis of rat skeletal muscle using 17521-1-AP (same clone as 17521-1-PBS)

    rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17521-1-AP (ACTA1-Specific Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17521-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using ACTA1-Specific Polyclonal antibody (17521-1-AP)

    IHC staining of human skeletal muscle using 17521-1-AP (same clone as 17521-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17521-1-AP (ACTA1-Specific Antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 17521-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using ACTA1-Specific Polyclonal antibody (17521-1-AP)

    IHC staining of human skeletal muscle using 17521-1-AP (same clone as 17521-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17521-1-AP (ACTA1-Specific Antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 17521-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using ACTA1-Specific Polyclonal antibody (17521-1-AP)

    IHC staining of human skeletal muscle using 17521-1-AP (same clone as 17521-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 17521-1-AP (ACTA1-Specific Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 17521-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using ACTA1-Specific Polyclonal antibody (17521-1-AP)

    IHC staining of human skeletal muscle using 17521-1-AP (same clone as 17521-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 17521-1-AP (ACTA1-Specific Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 17521-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human hysteromyoma tissue using ACTA1-Specific Polyclonal antibody (17521-1-AP)

    IHC staining of human hysteromyoma using 17521-1-AP (same clone as 17521-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hysteromyoma using 17521-1-AP (ACTA1-Specific antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17521-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human hysteromyoma tissue using ACTA1-Specific Polyclonal antibody (17521-1-AP)

    IHC staining of human hysteromyoma using 17521-1-AP (same clone as 17521-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hysteromyoma using 17521-1-AP (ACTA1-Specific antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17521-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of C2C12 cells using ACTA1-Specific Polyclonal antibody (17521-1-AP)

    FC experiment of C2C12 using 17521-1-AP (same clone as 17521-1-PBS)

    1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human ACTA1-Specific (17521-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 17521-1-PBS in a different storage buffer formulation.

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ACTA1-Specific Polyclonal antibody, PBS Only

ACTA1-Specific Polyclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 17521-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17521-1-PBS targets ACTA1-Specific in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name actin, alpha 1, skeletal muscle
Calculated molecular weight 42 kDa
Observed molecular weight 39-42 kDa
GenBank accession numberNM_001100
Gene Symbol ACTA1
Gene ID (NCBI) 58
RRIDAB_2273608
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP68133
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. The ACTA1 gene encodes skeletal muscle alpha-actin, the principal actin isoform in adult skeletal muscle, which interacts with a variety of proteins to produce the force for muscle contraction. There is only the N-termninal 8 amino acids difference among the actin family members. This antibody is specific to the ACTA1. It does not cross-react with other actin isoforms.