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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ACTA1-Specific Recombinant antibody, PBS Only

ACTA1-Specific Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat, pig, chicken

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5B15

Cat no : 82714-5-PBS

Synonyms

ACTA1, Alpha Actin, α actin, Actin, alpha skeletal muscle, Actin alpha skeletal muscle

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ACTA1-Specific Recombinant antibody (82714-5-RR)

    WB analysis using 82714-5-RR (same clone as 82714-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82714-5-RR (ACTA1-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82714-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using ACTA1-Specific Recombinant antibody (82714-5-RR)

    IF Staining of mouse skeletal muscle using 82714-5-RR (same clone as 82714-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using ACTA1-Specific antibody (82714-5-RR, Clone: 5B15 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82714-5-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82714-5-PBS targets ACTA1-Specific in WB, IF/ICC, Indirect ELISA applications and shows reactivity with mouse, rat, pig, chicken samples.

Tested Reactivity mouse, rat, pig, chicken
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name actin, alpha 1, skeletal muscle
Calculated molecular weight 42 kDa
Observed molecular weight 39-42 kDa
GenBank accession numberNM_001100
Gene symbol ACTA1
Gene ID (NCBI) 58
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. The ACTA1 gene encodes skeletal muscle alpha-actin, the principal actin isoform in adult skeletal muscle, which interacts with a variety of proteins to produce the force for muscle contraction. There is only the N-termninal 8 amino acids difference among the actin family members. This antibody is specific to the ACTA1. It does not cross-react with other actin isoforms.