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  • Featured Product
  • KD/KO Validated

smooth muscle actin Polyclonal antibody

smooth muscle actin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA, competitive binding assay

Conjugate

Unconjugated

Cat no : 14395-1-AP

Synonyms

ACTA2, Alpha SMA, a-SMA, Alpha actin 2, Actin, aortic smooth muscle, intermediate form

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using smooth muscle actin Polyclonal antibody (14395-1-AP)

    WB analysis using 14395-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14395-1-AP (smooth muscle actin antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using smooth muscle actin Polyclonal antibody (14395-1-AP)

    WB analysis using 14395-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14395-1-AP (smooth muscle actin antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using smooth muscle actin Polyclonal antibody (14395-1-AP)

    WB analysis of A549 using 14395-1-AP

    WB result of smooth muscle actin antibody (14395-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-smooth muscle actin transfected A549 cells.

  • Western Blot (WB) analysis of human placenta tissue using smooth muscle actin Polyclonal antibody (14395-1-AP)

    WB analysis of human placenta using 14395-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using smooth muscle actin Polyclonal antibody (14395-1-AP)

    IP experiment of mouse liver using 14395-1-AP

    IP result of anti-smooth muscle actin (IP:14395-1-AP, 3ug; Detection:14395-1-AP 1:1000) with mouse liver tissue lysate 6000ug.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using smooth muscle actin Polyclonal antibody (14395-1-AP)

    IHC staining of human liver cancer using 14395-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:3000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using smooth muscle actin Polyclonal antibody (14395-1-AP)

    IHC staining of human colon using 14395-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human heart tissue using smooth muscle actin Polyclonal antibody (14395-1-AP)

    IHC staining of human heart using 14395-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody at dilution of 1:5000 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using smooth muscle actin Polyclonal antibody (14395-1-AP)

    IHC staining of human heart using 14395-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody at dilution of 1:5000 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using smooth muscle actin Polyclonal antibody (14395-1-AP)

    IHC staining of human liver cancer using 14395-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using smooth muscle actin Polyclonal antibody (14395-1-AP)

    IHC staining of human liver cancer using 14395-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:10000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of stromal tumor tissue using smooth muscle actin Polyclonal antibody (14395-1-AP)

    IHC staining of stromal tumor using 14395-1-AP

    Immunohistochemical analysis of paraffin-embedded stromal tumor tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:10000 (under 10x lens).

  • Immunohistochemistry (IHC) staining of stromal tumor tissue using smooth muscle actin Polyclonal antibody (14395-1-AP)

    IHC staining of stromal tumor using 14395-1-AP

    Immunohistochemical analysis of paraffin-embedded stromal tumor tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:10000 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human hysteromyoma tissue using smooth muscle actin Polyclonal antibody (14395-1-AP)

    IHC staining of human hysteromyoma using 14395-1-AP

    Immunohistochemical analysis of paraffin-embedded human hysteromyoma tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human hysteromyoma tissue using smooth muscle actin Polyclonal antibody (14395-1-AP)

    IHC staining of human hysteromyoma using 14395-1-AP

    Immunohistochemical analysis of paraffin-embedded human hysteromyoma tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:10000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using smooth muscle actin Polyclonal antibody (14395-1-AP)

    IHC staining of human colon using 14395-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:10000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using smooth muscle actin Polyclonal antibody (14395-1-AP)

    IHC staining of human liver cancer using 14395-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:3000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using smooth muscle actin Polyclonal antibody (14395-1-AP)

    IF Staining of mouse heart using 14395-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 14395-1-AP (smooth muscle actin antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse small intestine tissue using smooth muscle actin Polyclonal antibody (14395-1-AP)

    IF Staining of mouse small intestine using 14395-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using smooth muscle actin antibody (14395-1-AP) at dilution of 1:4000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of C2C12 cells using smooth muscle actin Polyclonal antibody (14395-1-AP)

    IF Staining of C2C12 using 14395-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using smooth muscle actin antibody (14395-1-AP) at dilution of 1:1600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of C2C12 cells using smooth muscle actin Polyclonal antibody (14395-1-AP)

    IF Staining of C2C12 using 14395-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using smooth muscle actin antibody (14395-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of H9C2 cells using smooth muscle actin Polyclonal antibody (14395-1-AP)

    IF Staining of H9C2 using 14395-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed H9C2 cells using smooth muscle actin antibody (14395-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of C2C12 cells using smooth muscle actin Polyclonal antibody (14395-1-AP)

    FC experiment of C2C12 using 14395-1-AP

    1x10^6 C2C12 cells were intracellularly stained with 0.4 ug smooth muscle actin Polyclonal antibody (14395-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inmouse colon tissue, HUVEC cells, human placenta tissue, A549 cells, mouse liver tissue, mouse kidney tissue, rat colon tissue, mouse heart tissue, rat heart tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver cancer tissue, human heart tissue, stromal tumor tissue, human hysteromyoma tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse heart tissue, mouse small intestine tissue
Positive IF/ICC detected inC2C12 cells, H9C2 cells
Positive FC (Intra) detected inC2C12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1500-1:6000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:800-1:3200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14395-1-AP targets smooth muscle actin in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA, competitive binding assay applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, rabbit, canine, monkey, zebrafish, bovine, sheep
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen smooth muscle actin fusion protein Ag5886 相同性解析による交差性が予測される生物種
Full Name actin, alpha 2, smooth muscle, aorta
Calculated molecular weight 377 aa, 42 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC017554
Gene symbol Alpha smooth muscle actin
Gene ID (NCBI) 59
RRIDAB_2223009
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells. This antibody may cross-react with other isoforms of actins.

Protocols

Product Specific Protocols
WB protocol for smooth muscle actin antibody 14395-1-APDownload protocol
IHC protocol for smooth muscle actin antibody 14395-1-APDownload protocol
IF protocol for smooth muscle actin antibody 14395-1-APDownload protocol
IP protocol for smooth muscle actin antibody 14395-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIF

Adv Mater

Exoskeleton Partial-Coated Stem Cells for Infarcted Myocardium Restoring

Authors - Huihui He
View Article
mouse,pigWB

Cell Metab

Augmentation of scleral glycolysis promotes myopia through histone lactylation

Authors - Xiaolei Lin
View Article
mouseIF

Mil Med Res

Caveolin-1 is critical for hepatic iron storage capacity in the development of nonalcoholic fatty liver disease

Authors - Guang-Hui Deng
View Article
mouseWB

Nat Cell Biol

LncGata6 maintains stemness of intestinal stem cells and promotes intestinal tumorigenesis.

Authors - Pingping Zhu
View Article
rabbitIF

Bioact Mater

Step-wise CAG@PLys@PDA-Cu2+ modification on micropatterned nanofibers for programmed endothelial healing

Authors - Bingcheng Yi
View Article
IHC

ACS Nano

Endoscopy Deliverable and Mushroom-Cap-Inspired Hyperboloid-Shaped Drug-Laden Bioadhesive Hydrogel for Stomach Perforation Repair

Authors - Shuang Liu
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

James (Verified Customer) (09-22-2022)

Used by IF. Very well antibody.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1 to 200
  • Cell Tissue Type: kidney
smooth muscle actin Antibody Immunohistochemistry validation (1 to 200 dilution) in kidney (Cat no:14395-1-AP)
FH

Wu (Verified Customer) (09-09-2022)

Used by IF. Really good signal

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:150
  • Cell Tissue Type: fibroblast cell
smooth muscle actin Antibody Immunofluorescence validation (1:150 dilution) in fibroblast cell (Cat no:14395-1-AP)
FH

Emma (Verified Customer) (11-29-2021)

Used by IF on FFPE prostate tissue. Tris-EDTA pH 9.0 antigen retrieval.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: Prostate
smooth muscle actin Antibody Immunofluorescence validation (1:50 dilution) in Prostate (Cat no:14395-1-AP)
FH

Ryan (Verified Customer) (02-28-2019)

Tissue was fixed in PFA and antigen retrieval was done in NaCit ph=6.

  • Applications: Immunohistochemistry, Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Zebrafish ocular muscles at 3dpf