Validation Data Gallery

Filter: AllWBIPIHCIF-PIF/ICCFC (Intra)

WB analysis using 14395-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14395-1-AP (smooth muscle actin antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis using 14395-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14395-1-AP (smooth muscle actin antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 14395-1-AP
WB result of smooth muscle actin antibody (14395-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-smooth muscle actin transfected A549 cells.
WB analysis of human placenta using 14395-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IP experiment of mouse liver using 14395-1-AP
IP result of anti-smooth muscle actin (IP:14395-1-AP, 3ug; Detection:14395-1-AP 1:1000) with mouse liver tissue lysate 6000ug.
IHC staining of human liver cancer using 14395-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:3000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 14395-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 14395-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:3000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human heart using 14395-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody at dilution of 1:5000 (under 10x lens).
IHC staining of human appendicitis using 14395-1-AP
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 14395-1-AP (smooth muscle actin antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human appendicitis using 14395-1-AP
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 14395-1-AP (smooth muscle actin antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse colon using 14395-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 14395-1-AP (Alpha smooth muscle actin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse colon using 14395-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 14395-1-AP (Alpha smooth muscle actin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human heart using 14395-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody at dilution of 1:5000 (under 40x lens).
IHC staining of human colon using 14395-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:10000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 14395-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 14395-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:10000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of stromal tumor using 14395-1-AP
Immunohistochemical analysis of paraffin-embedded stromal tumor tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:10000 (under 10x lens).
IHC staining of stromal tumor using 14395-1-AP
Immunohistochemical analysis of paraffin-embedded stromal tumor tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:10000 (under 40x lens).
IHC staining of human hysteromyoma using 14395-1-AP
Immunohistochemical analysis of paraffin-embedded human hysteromyoma tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human hysteromyoma using 14395-1-AP
Immunohistochemical analysis of paraffin-embedded human hysteromyoma tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:10000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse small intestine using 14395-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using smooth muscle actin antibody (14395-1-AP) at dilution of 1:4000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse heart using 14395-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 14395-1-AP (smooth muscle actin antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of C2C12 using 14395-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using smooth muscle actin antibody (14395-1-AP) at dilution of 1:1600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of C2C12 using 14395-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using smooth muscle actin antibody (14395-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of H9C2 using 14395-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed H9C2 cells using smooth muscle actin antibody (14395-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of C2C12 using 14395-1-AP
1x10^6 C2C12 cells were intracellularly stained with 0.4 ug smooth muscle actin Polyclonal antibody (14395-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Featured Product
KD/KO Validated

Alpha smooth muscle actin Polyclonal antibody

Alpha smooth muscle actin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Cat No. 14395-1-AP

Publications(1261)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA, competitive binding assay

Synonyms

ACTA2, Alpha SMA, a-SMA, smooth muscle actin, Alpha actin 2

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®594

Size/Concentration: 150 μL, 550 μg/ml

20 μL, 550 μg/ml

150 μL, 550 μg/ml

¥67,000/ 150 μL

本製品の国内在庫をすぐに見る（表示に数秒かかります）

【全製品対象☆】小さなギフトがついてくる！春の特典キャンペーン開催中

他社製品の代替品をお探しですか？『抗体代替品／同等品検索システム』でお調べいただけます

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (1261)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse colon tissue, human placenta tissue, A549 cells, HUVEC cells, rat colon tissue, mouse heart tissue, rat heart tissue, mouse liver tissue, mouse kidney tissue
Positive IP detected in	mouse liver tissue
Positive IHC detected in	human liver cancer tissue, human heart tissue, human colon tissue, human hysteromyoma tissue, stromal tumor tissue, human appendicitis tissue, mouse colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse heart tissue, mouse small intestine tissue
Positive IF/ICC detected in	C2C12 cells, H9C2 cells
Positive FC (Intra) detected in	C2C12 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:1500-1:6000
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:800-1:3200
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 832 publications below
IHC	See 363 publications below
IF	See 500 publications below
FC	See 3 publications below
CoIP	See 1 publications below

Product Information

14395-1-AP targets Alpha smooth muscle actin in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA, competitive binding assay applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, rabbit, canine, monkey, zebrafish, bovine, sheep
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Alpha smooth muscle actin fusion protein Ag5886 相同性解析による交差性が予測される生物種
Full Name	actin, alpha 2, smooth muscle, aorta
Calculated molecular weight	377 aa, 42 kDa
Observed molecular weight	42 kDa
GenBank accession number	BC017554
Gene Symbol	Alpha smooth muscle actin
Gene ID (NCBI)	59
RRID	AB_2223009
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P62736
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells. This antibody may cross-react with other isoforms of actins.

Protocols

Product Specific Protocols
WB protocol for Alpha smooth muscle actin antibody 14395-1-AP	Download protocol
IHC protocol for Alpha smooth muscle actin antibody 14395-1-AP	Download protocol
IF protocol for Alpha smooth muscle actin antibody 14395-1-AP	Download protocol
IP protocol for Alpha smooth muscle actin antibody 14395-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
rat	IF	Adv Mater Exoskeleton Partial-Coated Stem Cells for Infarcted Myocardium Restoring Authors - Huihui He View Article
mouse,pig	WB	Cell Metab Augmentation of scleral glycolysis promotes myopia through histone lactylation Authors - Xiaolei Lin View Article
mouse	IF	Mil Med Res Caveolin-1 is critical for hepatic iron storage capacity in the development of nonalcoholic fatty liver disease Authors - Guang-Hui Deng View Article
mouse	WB	Nat Cell Biol LncGata6 maintains stemness of intestinal stem cells and promotes intestinal tumorigenesis. Authors - Pingping Zhu View Article
rabbit	IF	Bioact Mater Step-wise CAG@PLys@PDA-Cu2+ modification on micropatterned nanofibers for programmed endothelial healing Authors - Bingcheng Yi View Article
	IHC	ACS Nano Endoscopy Deliverable and Mushroom-Cap-Inspired Hyperboloid-Shaped Drug-Laden Bioadhesive Hydrogel for Stomach Perforation Repair Authors - Shuang Liu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 14395-1-AP

WB analysis using 14395-1-AP

WB analysis of A549 using 14395-1-AP

WB analysis of human placenta using 14395-1-AP

IP experiment of mouse liver using 14395-1-AP

IHC staining of human liver cancer using 14395-1-AP

IHC staining of human colon using 14395-1-AP

IHC staining of human liver cancer using 14395-1-AP

IHC staining of human heart using 14395-1-AP

IHC staining of human appendicitis using 14395-1-AP

IHC staining of human appendicitis using 14395-1-AP

IHC staining of mouse colon using 14395-1-AP

IHC staining of mouse colon using 14395-1-AP

IHC staining of human heart using 14395-1-AP

IHC staining of human colon using 14395-1-AP

IHC staining of human liver cancer using 14395-1-AP

IHC staining of human liver cancer using 14395-1-AP

IHC staining of stromal tumor using 14395-1-AP

IHC staining of stromal tumor using 14395-1-AP

IHC staining of human hysteromyoma using 14395-1-AP

IHC staining of human hysteromyoma using 14395-1-AP

IF Staining of mouse small intestine using 14395-1-AP

IF Staining of mouse heart using 14395-1-AP

IF Staining of C2C12 using 14395-1-AP

IF Staining of C2C12 using 14395-1-AP

IF Staining of H9C2 using 14395-1-AP

FC experiment of C2C12 using 14395-1-AP

Recommended products:

Multi-rAb Recombinant Secondary Antibodies

New FlexAble 2.0 Antibody Labeling Kits

Alpha smooth muscle actin Polyclonal antibody

Alpha smooth muscle actin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

¥67,000/ 150 μL

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Exoskeleton Partial-Coated Stem Cells for Infarcted Myocardium Restoring

Augmentation of scleral glycolysis promotes myopia through histone lactylation

Caveolin-1 is critical for hepatic iron storage capacity in the development of nonalcoholic fatty liver disease

LncGata6 maintains stemness of intestinal stem cells and promotes intestinal tumorigenesis.

Step-wise CAG@PLys@PDA-Cu2+ modification on micropatterned nanofibers for programmed endothelial healing

Endoscopy Deliverable and Mushroom-Cap-Inspired Hyperboloid-Shaped Drug-Laden Bioadhesive Hydrogel for Stomach Perforation Repair

^NewFlexAble 2.0 Antibody Labeling Kits