Alpha smooth muscle actin Polyclonal antibody

Name: Alpha smooth muscle actin Polyclonal antibody
Brand: Proteintech
SKU: 23081-1-AP

Alpha smooth muscle actin Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 23081-1-AP

Synonyms

ACTA2, Alpha SMA, a-SMA, smooth muscle actin, Alpha actin 2

Validation Data Gallery

Filter:

AllWBIPIHC

WB analysis of mouse heart using 23081-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brown adipose using 23081-1-AP
mouse brown adipose tissue were subjected to SDS PAGE followed by western blot with 23081-1-AP (a-SMA specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat heart using 23081-1-AP
rat heart tissue were subjected to SDS PAGE followed by western blot with 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 23081-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 23081-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 23081-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse heart using 23081-1-AP
IP result of anti-smooth muscle actin (IP:23081-1-AP, 4ug; Detection:23081-1-AP 1:1000) with mouse heart tissue lysate 3200ug.
IHC staining of human heart using 23081-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:500 (under 10x lens).
IHC staining of human colon using 23081-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:6400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 23081-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:3200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human heart using 23081-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:500 (under 40x lens).
IHC staining of mouse skeletal muscle using 23081-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse skeletal muscle using 23081-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skeletal muscle using 23081-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:500 (under 10x lens).
IHC staining of human skeletal muscle using 23081-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:500 (under 40x lens).
IHC staining of human liver cancer using 23081-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:3200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of stromal tumor using 23081-1-AP
Immunohistochemical analysis of paraffin-embedded stromal tumor tissue slide using 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:3200 (under 10x lens).
IHC staining of stromal tumor using 23081-1-AP
Immunohistochemical analysis of paraffin-embedded stromal tumor tissue slide using 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:3200 (under 40x lens).
IHC staining of human hysteromyoma using 23081-1-AP
Immunohistochemical analysis of paraffin-embedded human hysteromyoma tissue slide using 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:3200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human hysteromyoma using 23081-1-AP
Immunohistochemical analysis of paraffin-embedded human hysteromyoma tissue slide using 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:3200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 23081-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:6400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human small intestine using 23081-1-AP
Immunohistochemical analysis of paraffin-embedded human small intestine using 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:500 (under 10x lens).
IHC staining of human small intestine using 23081-1-AP
Immunohistochemical analysis of paraffin-embedded human small intestine using 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:500 (under 40x lens).
IHC staining of human liver using 23081-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 23081-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 23081-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse heart tissue, mouse brown adipose tissue, mouse kidney tissue, mouse liver tissue, mouse skeletal muscle tissue, rat heart tissue
Positive IP detected in	mouse heart tissue
Positive IHC detected in	human heart tissue, mouse skeletal muscle tissue, human colon tissue, human liver cancer tissue, stromal tumor tissue, human hysteromyoma tissue, human liver tissue, human small intestine tissue, human skeletal muscle tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 21 publications below
IHC	See 11 publications below
IF	See 7 publications below

Product Information

23081-1-AP targets Alpha smooth muscle actin in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Alpha smooth muscle actin fusion protein Ag19481 相同性解析による交差性が予測される生物種
Full Name	actin, alpha 2, smooth muscle, aorta
Calculated molecular weight	377 aa, 42 kDa
Observed molecular weight	42 kDa
GenBank accession number	BC017554
Gene symbol	Alpha smooth muscle actin
Gene ID (NCBI)	59
RRID	AB_2815024
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells. This antibody is specific to the ACTA2. It selectively stains the vascular smooth muscle but not cardiac or skeletal muscle.

Protocols

Product Specific Protocols
WB protocol for Alpha smooth muscle actin antibody 23081-1-AP	Download protocol
IHC protocol for Alpha smooth muscle actin antibody 23081-1-AP	Download protocol
IP protocol for Alpha smooth muscle actin antibody 23081-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Nat Commun Dental cell type atlas reveals stem and differentiated cell types in mouse and human teeth. Authors - Jan Krivanek View Article
human	WB	Drug Des Devel Ther Keloid Patient Plasma-Derived Exosomal hsa_circ_0020792 Promotes Normal Skin Fibroblasts Proliferation, Migration, and Fibrogenesis via Modulating miR-193a-5p and Activating TGF-β1/Smad2/3 Signaling Authors - Huan Hu View Article
mouse	WB	Biofabrication Antibacterial, ROS scavenging and angiogenesis promotingϵ-Polylysine/gelatin based hydrogel containing CTLP to regulate macrophages for pressure ulcer healing Authors - PinLi Lin View Article
mouse	IHC	Cardiovasc Res Plasma levels of trimethylamine-N-oxide can be increased with 'healthy' and 'unhealthy' diets and do not correlate with the extent of atherosclerosis but with plaque instability. Authors - Yen Chin Koay View Article
rat	WB	Environ Pollut Polystyrene microplastics cause cardiac fibrosis by activating Wnt/β-catenin signaling pathway and promoting cardiomyocyte apoptosis in rats. Authors - Zekang Li View Article
rat	IHC	Cardiovasc Res PMID: 23650288 Authors - Fan You-Fei YF View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Alpha smooth muscle actin Polyclonal antibody

Alpha smooth muscle actin Polyclonal Antibody for WB, IHC, IP, ELISA

Validation Data Gallery

WB analysis of mouse heart using 23081-1-AP

WB analysis of mouse brown adipose using 23081-1-AP

WB analysis of rat heart using 23081-1-AP

WB analysis of mouse skeletal muscle using 23081-1-AP

WB analysis of mouse kidney using 23081-1-AP

WB analysis of mouse liver using 23081-1-AP

IP experiment of mouse heart using 23081-1-AP

IHC staining of human heart using 23081-1-AP

IHC staining of human colon using 23081-1-AP

IHC staining of human liver cancer using 23081-1-AP

IHC staining of human heart using 23081-1-AP

IHC staining of mouse skeletal muscle using 23081-1-AP

IHC staining of mouse skeletal muscle using 23081-1-AP

IHC staining of human skeletal muscle using 23081-1-AP

IHC staining of human skeletal muscle using 23081-1-AP

IHC staining of human liver cancer using 23081-1-AP

IHC staining of stromal tumor using 23081-1-AP

IHC staining of stromal tumor using 23081-1-AP

IHC staining of human hysteromyoma using 23081-1-AP

IHC staining of human hysteromyoma using 23081-1-AP

IHC staining of human colon using 23081-1-AP

IHC staining of human small intestine using 23081-1-AP

IHC staining of human small intestine using 23081-1-AP

IHC staining of human liver using 23081-1-AP

IHC staining of human liver using 23081-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Dental cell type atlas reveals stem and differentiated cell types in mouse and human teeth.

Keloid Patient Plasma-Derived Exosomal hsa_circ_0020792 Promotes Normal Skin Fibroblasts Proliferation, Migration, and Fibrogenesis via Modulating miR-193a-5p and Activating TGF-β1/Smad2/3 Signaling

Antibacterial, ROS scavenging and angiogenesis promotingϵ-Polylysine/gelatin based hydrogel containing CTLP to regulate macrophages for pressure ulcer healing

Plasma levels of trimethylamine-N-oxide can be increased with 'healthy' and 'unhealthy' diets and do not correlate with the extent of atherosclerosis but with plaque instability.

Polystyrene microplastics cause cardiac fibrosis by activating Wnt/β-catenin signaling pathway and promoting cardiomyocyte apoptosis in rats.

PMID: 23650288

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