Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis using 20536-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 20536-1-AP (beta Actin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of A549 cells using 20536-1-AP
A549 cells (shcontrol and shRNA of Beta actin) were subjected to SDS PAGE followed by western blot with 20536-1-AP (ACTB antibody) at dilution of 1:500.
WB analysis using 20536-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 20536-1-AP (Beta Actin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 20536-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 20536-1-AP (beta Actin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 20536-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 20536-1-AP (beta Actin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 20536-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 20536-1-AP (beta Actin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of C6 using 20536-1-AP
C6 cells were subjected to SDS PAGE followed by western blot with 20536-1-AP (beta actin Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC staining of human colon using 20536-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 20536-1-AP (Beta Actin antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 20536-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 20536-1-AP (Beta Actin antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 20536-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20536-1-AP (beta actin Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human kidney using 20536-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20536-1-AP (beta actin Antibody) at dilution of 1:200 (under 40x lens).
IF Staining of MDCK using 20536-1-AP
Immunofluorescent analysis of (4% PFA) fixed MDCK cells using Beta Actin antibody (20536-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Featured Product
KD/KO Validated

Beta Actin Polyclonal antibody

Beta Actin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 20536-1-AP

Publications(4230)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine, monkey and More (7)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

Actin, ACTB, bate actin, β actin, Beta-actin

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 647

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (4230)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, A549 cells, C6 cells, mouse colon tissue, Caco-2 cells, RAW 264.7 cells, SMMC-7721 cells, HeLa cells, HepG2 cells, Jurkat cells, mouse brain tissue, rat brain tissue, NIH/3T3 cells, mouse liver tissue, rat kidney tissue, rat spleen tissue, rat liver tissue
Positive IHC detected in	human colon tissue, human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MDCK cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:4000-1:10000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 4109 publications below
IHC	See 4 publications below
IF	See 14 publications below
IP	See 8 publications below
ELISA	See 1 publications below
CoIP	See 2 publications below

Product Information

20536-1-AP targets Beta Actin in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, canine, monkey samples.

Tested Reactivity	human, mouse, rat, canine, monkey
Cited Reactivity	human, mouse, rat, chicken, goat, sheep, yeast, ochotona curzoniae, camelus bactrianus, carp
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Beta Actin fusion protein Ag14521 相同性解析による交差性が予測される生物種
Full Name	actin, beta
Calculated molecular weight	375 aa, 42 kDa
Observed molecular weight	42 kDa
GenBank accession number	BC002409
Gene Symbol	Beta Actin
Gene ID (NCBI)	60
RRID	AB_10700003
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P60709
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Beta Actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 37-40, 31, 15 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody was generated against N-terminal region of human beta actin protein and can cross-react with other actins. (9173887, 11217076, 10229193 )

Protocols

Product Specific Protocols
WB protocol for Beta Actin antibody 20536-1-AP	Download protocol
IHC protocol for Beta Actin antibody 20536-1-AP	Download protocol
IF protocol for Beta Actin antibody 20536-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Nature A plant-derived natural photosynthetic system for improving cell anabolism Authors - Pengfei Chen View Article
		Nature Promotion of DNA end resection by BRCA1-BARD1 in homologous recombination Authors - Sameer Salunkhe View Article
human	WB	Cancer Cell Targeting the immune privilege of tumor-initiating cells to enhance cancer immunotherapy Authors - Chen Yang View Article
mouse	WB	Nature Aspm knockout ferret reveals an evolutionary mechanism governing cerebral cortical size. Authors - Matthew B Johnson View Article
		Signal Transduct Target Ther Identifying genetic targets in clinical subtypes of Parkinson's disease for optimizing pharmacological treatment strategies Authors - Dewen Kong View Article
	WB	Cell Microglia jointly degrade fibrillar alpha-synuclein cargo by distribution through tunneling nanotubes. Authors - Hannah Scheiblich View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 20536-1-AP

WB analysis of A549 cells using 20536-1-AP

WB analysis using 20536-1-AP

WB analysis using 20536-1-AP

WB analysis using 20536-1-AP

WB analysis using 20536-1-AP

WB analysis of C6 using 20536-1-AP

IHC staining of human colon using 20536-1-AP

IHC staining of human colon using 20536-1-AP

IHC staining of human kidney using 20536-1-AP

IHC staining of human kidney using 20536-1-AP

IF Staining of MDCK using 20536-1-AP

Beta Actin Polyclonal antibody

Beta Actin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

A plant-derived natural photosynthetic system for improving cell anabolism

Promotion of DNA end resection by BRCA1-BARD1 in homologous recombination

Targeting the immune privilege of tumor-initiating cells to enhance cancer immunotherapy

Aspm knockout ferret reveals an evolutionary mechanism governing cerebral cortical size.

Identifying genetic targets in clinical subtypes of Parkinson's disease for optimizing pharmacological treatment strategies

Microglia jointly degrade fibrillar alpha-synuclein cargo by distribution through tunneling nanotubes.