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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Alpha Actin Polyclonal antibody

Alpha Actin Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 23082-1-AP

Synonyms

ACTC1, Actin, alpha cardiac muscle 1, actin, ACTC1 cardiac muscle 1, ACTC 1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse heart tissue using Alpha Actin Polyclonal antibody (23082-1-AP)

    WB analysis of mouse heart using 23082-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 23082-1-AP (alpha Actin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human heart tissue using Alpha Actin Polyclonal antibody (23082-1-AP)

    IHC staining of human heart using 23082-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 23082-1-AP (alpha Actin antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using Alpha Actin Polyclonal antibody (23082-1-AP)

    IHC staining of human heart using 23082-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 23082-1-AP (alpha Actin antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using Alpha Actin Polyclonal antibody (23082-1-AP)

    IHC staining of human colon using 23082-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 23082-1-AP (Alpha Actin antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Alpha Actin Polyclonal antibody (23082-1-AP)

    IHC staining of mouse heart using 23082-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 23082-1-AP (alpha Actin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using Alpha Actin Polyclonal antibody (23082-1-AP)

    IHC staining of mouse colon using 23082-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 23082-1-AP (alpha Actin antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using Alpha Actin Polyclonal antibody (23082-1-AP)

    IHC staining of mouse colon using 23082-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 23082-1-AP (alpha Actin antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using Alpha Actin Polyclonal antibody (23082-1-AP)

    IHC staining of human colon using 23082-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 23082-1-AP (alpha Actin antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using Alpha Actin Polyclonal antibody (23082-1-AP)

    IHC staining of human colon using 23082-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 23082-1-AP (alpha Actin antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using Alpha Actin Polyclonal antibody (23082-1-AP)

    IHC staining of human skeletal muscle using 23082-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 23082-1-AP (alpha Actin antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using Alpha Actin Polyclonal antibody (23082-1-AP)

    IHC staining of human skeletal muscle using 23082-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 23082-1-AP (alpha Actin antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using Alpha Actin Polyclonal antibody (23082-1-AP)

    IHC staining of human small intestine using 23082-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine using 23082-1-AP (alpha Actin antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using Alpha Actin Polyclonal antibody (23082-1-AP)

    IHC staining of human small intestine using 23082-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine using 23082-1-AP (alpha Actin antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using Alpha Actin Polyclonal antibody (23082-1-AP)

    IHC staining of human skeletal muscle using 23082-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 23082-1-AP (alpha Actin antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using Alpha Actin Polyclonal antibody (23082-1-AP)

    IHC staining of human skeletal muscle using 23082-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 23082-1-AP (alpha Actin antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using Alpha Actin Polyclonal antibody (23082-1-AP)

    IHC staining of mouse skeletal muscle using 23082-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 23082-1-AP (alpha Actin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using Alpha Actin Polyclonal antibody (23082-1-AP)

    IHC staining of mouse skeletal muscle using 23082-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 23082-1-AP (alpha Actin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Alpha Actin Polyclonal antibody (23082-1-AP)

    IHC staining of mouse heart using 23082-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 23082-1-AP (alpha Actin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human heart tissue using Alpha Actin Polyclonal antibody (23082-1-AP)

    IHC staining of human heart using 23082-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 23082-1-AP (alpha Actin antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using Alpha Actin Polyclonal antibody (23082-1-AP)

    IHC staining of human heart using 23082-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 23082-1-AP (alpha Actin antibody) at dilution of 1:50 (under 40x lens).

  • Flow cytometry (FC) experiment of C2C12 cells using Alpha Actin Polyclonal antibody (23082-1-AP)

    FC experiment of C2C12 using 23082-1-AP

    1x10^6 C2C12 cells were intracellularly stained with 0.4 ug Alpha Actin Polyclonal antibody (23082-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inmouse heart tissue
Positive IHC detected inhuman normal colon, human colon tissue, human heart tissue, human skeletal muscle tissue, human small intestine tissue, mouse skeletal muscle tissue, mouse heart tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inC2C12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

23082-1-AP targets Alpha Actin in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Alpha Actin fusion protein Ag19482 相同性解析による交差性が予測される生物種
Full Name actin, alpha, cardiac muscle 1
Calculated molecular weight 377 aa, 42 kDa
Observed molecular weight 42-45 kDa
GenBank accession numberBC009978
Gene symbol ACTC1
Gene ID (NCBI) 70
RRIDAB_2879206
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Alpha Actin antibody 23082-1-APDownload protocol
IHC protocol for Alpha Actin antibody 23082-1-APDownload protocol
FC protocol for Alpha Actin antibody 23082-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Circ Heart Fail

Phenotype and Physiological Significance of the Endocardial Smooth Muscle Cells in Human Failing Hearts.

Authors - Hideshi Okada
View Article