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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ACTC1-specific Monoclonal antibody, PBS Only

ACTC1-specific Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F2B9

Cat no : 66125-1-PBS

Synonyms

ACTC1, Alpha actin, actin, ACTC1 cardiac muscle 1, ACTC 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ACTC1-specific Monoclonal antibody (66125-1-Ig)

    WB analysis using 66125-1-Ig (same clone as 66125-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66125-1-Ig (ACTC1-specific antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66125-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using ACTC1-specific Monoclonal antibody (66125-1-Ig)

    WB analysis of human heart using 66125-1-Ig (same clone as 66125-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 66125-1-Ig (ACTC1-specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66125-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using ACTC1-specific Monoclonal antibody (66125-1-Ig)

    IHC staining of human heart using 66125-1-Ig (same clone as 66125-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 66125-1-Ig(ACTC1-specific antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66125-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using ACTC1-specific Monoclonal antibody (66125-1-Ig)

    IHC staining of human heart using 66125-1-Ig (same clone as 66125-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 66125-1-Ig(ACTC1-specific antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66125-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart and human skeletal muscle tissue using ACTC1-specific Monoclonal antibody (66125-1-Ig)

    IHC staining of human heart and human skeletal muscle tissue using 66125-1-Ig (same clone as 66125-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart and human skeletal muscle using 66125-1-Ig (ACTC1 specific antibody) at dilution 1:500. (under 40x lens). This data was developed using the same antibody clone with 66125-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using ACTC1-specific Monoclonal antibody (66125-1-Ig)

    IHC staining of human heart using 66125-1-Ig (same clone as 66125-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 66125-1-Ig(ACTC1-specific antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 66125-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using ACTC1-specific Monoclonal antibody (66125-1-Ig)

    IHC staining of human heart using 66125-1-Ig (same clone as 66125-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 66125-1-Ig(ACTC1-specific antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 66125-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using ACTC1-specific Monoclonal antibody (66125-1-Ig)

    IHC staining of human heart using 66125-1-Ig (same clone as 66125-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 66125-1-Ig(ACTC1-specific antibody) at dilution of 1:1000 (under 10x lens). This data was developed using the same antibody clone with 66125-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using ACTC1-specific Monoclonal antibody (66125-1-Ig)

    IHC staining of human heart using 66125-1-Ig (same clone as 66125-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 66125-1-Ig(ACTC1-specific antibody) at dilution of 1:1000 (under 40x lens). This data was developed using the same antibody clone with 66125-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using ACTC1-specific Monoclonal antibody (66125-1-Ig)

    IF Staining of mouse heart using 66125-1-Ig (same clone as 66125-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using ACTC1-specific antibody (66125-1-Ig, Clone: 1F2B9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66125-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of C2C12 cells using ACTC1-specific Monoclonal antibody (66125-1-Ig)

    FC experiment of C2C12 using 66125-1-Ig (same clone as 66125-1-PBS)

    1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human ACTC1-specific (66125-1-Ig, Clone:1F2B9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66125-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66125-1-PBS targets ACTC1-specific in WB, IHC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name actin, alpha, cardiac muscle 1
Calculated molecular weight 42 kDa
Observed molecular weight 42 kDa
GenBank accession numberNM_005159
Gene symbol ACTC1
Gene ID (NCBI) 70
RRIDAB_2881524
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. The ACTC1 gene encodes cardiac muscle alpha-actin, the predominant actin isoform in adult heart, which interacts with a variety of proteins to produce the force for muscle contraction. This antibody is specific to the ACTC1. It does not cross-react with other actin isoforms.