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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ACTG2 Polyclonal antibody (31849-1-AP)

    WB analysis using 31849-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31849-1-AP (ACTG2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ACTG2 Polyclonal antibody (31849-1-AP)

    WB analysis using 31849-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31849-1-AP (ACTG2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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ACTG2 Polyclonal antibody

ACTG2 Polyclonal Antibody for WB, ELISA
Cat No. 31849-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

ACTA3, Actin, gamma-enteric smooth muscle, Actin, gamma-enteric smooth muscle, intermediate form, ACTL3, ACTSG

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, mouse skeletal muscle tissue, HepG2 cells, mouse stomach tissue, rat skeletal muscle tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31849-1-AP targets ACTG2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACTG2 fusion protein Ag36024 相同性解析による交差性が予測される生物種
Full Name actin, gamma 2, smooth muscle, enteric
Calculated molecular weight42 kDa
Observed molecular weight42 kDa
GenBank accession numberBC094877
Gene Symbol ACTG2
Gene ID (NCBI) 72
RRIDAB_3670128
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP63267
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Actin gamma 2 (ACTG2, also known as ACT, ACTE, VSCM, ACTA3, ACTL3, and ACTSG) is a highly conserved protein involved in various types of cell motility and ubiquitously expressed in all eukaryotic cells. ACTG2 mutations would cause congenital distended bladder, microcolon, and intestinal hypoperistalsis (PMID: 24337657). It also has been reported that ACTG2 promotes hepatocellular carcinoma (HCC) migration and invasion through the Notch1 signaling pathway, which might serve as a new intervention target for the treatment of HCC (PMID: 28385530).

Protocols

Product Specific Protocols
WB protocol for ACTG2 antibody 31849-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols