ACTL7A Polyclonal antibody
ACTL7A Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IF, IHC, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 17355-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse testis tissue, rat testis tissue |
Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:5000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 7 publications below |
IHC | See 1 publications below |
IF | See 10 publications below |
IP | See 2 publications below |
CoIP | See 3 publications below |
Product Information
17355-1-AP targets ACTL7A in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | ACTL7A fusion protein Ag10613 相同性解析による交差性が予測される生物種 |
Full Name | actin-like 7A |
Calculated molecular weight | 435 aa, 49 kDa |
Observed molecular weight | 49 kDa |
GenBank accession number | BC014610 |
Gene symbol | ACTL7A |
Gene ID (NCBI) | 10881 |
RRID | AB_2273677 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ACTL7a is located in the acrosome and tail of mature spermatozoa and plays an important role in spermiogenesis. Recently mutation of ACTL7A has been linked to infertility.
Protocols
Product Specific Protocols | |
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WB protocol for ACTL7A antibody 17355-1-AP | Download protocol |
IHC protocol for ACTL7A antibody 17355-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Am J Hum Genet Homozygous pathogenic variants in ACTL9 cause fertilization failure and male infertility in humans and mice. | ||
Hum Reprod ACROSIN deficiency causes total fertilization failure in humans by preventing the sperm from penetrating the zona pellucida | ||
Hum Reprod Deletion of ACTRT1 is associated with male infertility as sperm acrosomal ultrastructural defects and fertilization failure in human | ||
Hum Reprod Loss of SPACA1 function causes autosomal recessive globozoospermia by damaging the acrosome-acroplaxome complex. | ||
Hum Reprod Novel bi-allelic variants in ACTL7A are associated with male infertility and total fertilization failure. | ||
Development Loss of perinuclear theca ACTRT1 causes acrosome detachment and severe male subfertility in mice. |