Validation Data Gallery
Tested Applications
| Positive WB detected in | human testis tissue, human liver tissue, mouse testis tissue |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
13537-1-AP targets ACTL7B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ACTL7B fusion protein Ag4460 相同性解析による交差性が予測される生物種 |
| Full Name | actin-like 7B |
| Calculated molecular weight | 415 aa, 45 kDa |
| Observed molecular weight | 45 kDa |
| GenBank accession number | BC033789 |
| Gene Symbol | ACTL7B |
| Gene ID (NCBI) | 10880 |
| RRID | AB_2223776 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y614 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ACTL7B is also named as actin-like-7-beta. The protein encoded by this gene is a member of a family of actin-related proteins (ARPs) which share significant amino acid sequence identity to conventional actins. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. The ACTL7B gene is expressed predominantly in the testis, however, its exact function is not known. Diseases associated with ACTL7B include Dysautonomia and Hypertensive Nephropathy. An important paralog of this gene is ACTL7A. ACTL7B can be detected as 45 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ACTL7B antibody 13537-1-AP | Download protocol |
| IHC protocol for ACTL7B antibody 13537-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Rep The Mammalian Spermatogenesis Single-Cell Transcriptome, from Spermatogonial Stem Cells to Spermatids. | ||
Development Actl7b deficiency leads to mislocalization of LC8 type dynein light chains and disruption of murine spermatogenesis |