ACTN2 Polyclonal antibody

Name: ACTN2 Polyclonal antibody
Brand: Proteintech
SKU: 14221-1-AP
Rating: 5.00 (1 reviews)

ACTN2 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Conjugate

Unconjugated

Publications

Reviews (1)

Cat no : 14221-1-AP

Synonyms

Alpha-actinin-2, Alpha-actinin skeletal muscle isoform 2, Alpha actinin 2, ACTN 2, actinin, alpha 2

Validation Data Gallery

Filter:

AllWBIPIHCIF-PIF/ICC

WB analysis using 14221-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14221-1-AP (ACTN2 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 14221-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 14221-1-AP (ACTN2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of C2C12 using 14221-1-AP
C2C12 cell were subjected to SDS PAGE followed by western blot with 14221-1-AP (ACTN2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 14221-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14221-1-AP (ACTN2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 14221-1-AP
IP result of anti-ACTN2 (IP:14221-1-AP, 4ug; Detection:14221-1-AP 1:1000) with HeLa cells lysate 1080ug.
IHC staining of mouse heart using 14221-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 14221-1-AP (ACTN2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse heart using 14221-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 14221-1-AP (ACTN2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse skeletal muscle using 14221-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14221-1-AP (ACTN2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse skeletal muscle using 14221-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14221-1-AP (ACTN2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse heart using 14221-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 14221-1-AP (ACTN2 antibody) at dilution of 1:400 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse heart using 14221-1-AP
Mouse cardiac tissue slice of 300µm thickness labelled with alpha actinin (14221-1-AP). Image courtesy of Chandan Kadur Nagaraju, Experimental Cardiology, KU Leuven, Belgium.
IF Staining of Human iPSC derived cardiomyocyte using 14221-1-AP
Human iPSC derived cardiomyocyte in 2D culture, with alpha actinin (14221-1-AP) and DAPI. Image courtesy of Chandan Kadur Nagaraju, Experimental Cardiology, KU Leuven, Belgium.
IF Staining of C2C12 using 14221-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed C2C12 cells using ACTN2 antibody (14221-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), 594phalloidin (red).
IF Staining of NIH/3T3 using 14221-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed NIH/3T3 cells using 14221-1-AP (ACTN2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of C2C12 using 14221-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed C2C12 cells using 14221-1-AP (ACTN2 antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 14221-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse brain tissue, C2C12 cells, mouse lung tissue, mouse skeletal muscle tissue, mouse heart, mouse kidney, rat skeletal muscle
Positive IP detected in	HeLa cells
Positive IHC detected in	mouse heart tissue, mouse skeletal muscle tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse heart tissue
Positive IF/ICC detected in	C2C12 cells, NIH/3T3 cells, Human iPSC derived cardiomyocyte

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)-P	IF-P : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 13 publications below
IHC	See 2 publications below
IF	See 8 publications below

Product Information

14221-1-AP targets ACTN2 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, chicken
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ACTN2 fusion protein Ag5459 相同性解析による交差性が予測される生物種
Full Name	actinin, alpha 2
Calculated molecular weight	104 kDa
Observed molecular weight	103 kDa
GenBank accession number	BC051770
Gene symbol	ACTN2
Gene ID (NCBI)	88
RRID	AB_2221547
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Alpha actinin 2 (ACTN2) belongs to the alpha-actinin family and is expressed in both skeletal and cardiac muscles and functions to anchor myofibrillar actin thin filaments and titin to Z-discs (PMID: 30701273). ACTN2 is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. Mutations in ACTN2 are associated with hypertrophic cardiomyopathy, as well as dilated cardiomyopathy and endocardial fibroelastosis (PMID: 20022194, 14567970).

Protocols

Product Specific Protocols
WB protocol for ACTN2 antibody 14221-1-AP	Download protocol
IHC protocol for ACTN2 antibody 14221-1-AP	Download protocol
IF protocol for ACTN2 antibody 14221-1-AP	Download protocol
IP protocol for ACTN2 antibody 14221-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
rat		Adv Mater Exoskeleton Partial-Coated Stem Cells for Infarcted Myocardium Restoring Authors - Huihui He View Article
mouse	WB,IF	Nat Commun Microskeletal stiffness promotes aortic aneurysm by sustaining pathological vascular smooth muscle cell mechanosensation via Piezo1. Authors - Weiyi Qian View Article
rat	WB	Basic Res Cardiol Alkaline nucleoplasm facilitates contractile gene expression in the mammalian heart. Authors - Alzbeta Hulikova View Article
human	IF	Dev Cell Essential role of MESP1-RING1A complex in cardiac differentiation Authors - Qianqian Liang View Article
human	IF	Front Cell Dev Biol Targeting of CAT and VCAM1 as Novel Therapeutic Targets for DMD Cardiomyopathy. Authors - Bin Li View Article
human	WB,IF	Front Cell Dev Biol Incomplete Assembly of the Dystrophin-Associated Protein Complex in 2D and 3D-Cultured Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes. Authors - Guillaume Gilbert View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

Rebecca (Verified Customer) (09-21-2022)

20ug of protein was loaded. Transference at 4ºC and 90V, for 2h. Antibody incubation ON at 4ºC.

Applications: Western Blot
Primary Antibody Dilution: 1:10000
Cell Tissue Type: iPSC-CM

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ACTN2 Polyclonal antibody

ACTN2 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Validation Data Gallery

WB analysis using 14221-1-AP

WB analysis of mouse lung using 14221-1-AP

WB analysis of C2C12 using 14221-1-AP

WB analysis of mouse skeletal muscle using 14221-1-AP

IP experiment of HeLa using 14221-1-AP

IHC staining of mouse heart using 14221-1-AP

IHC staining of mouse heart using 14221-1-AP

IHC staining of mouse skeletal muscle using 14221-1-AP

IHC staining of mouse skeletal muscle using 14221-1-AP

IF Staining of mouse heart using 14221-1-AP

IF Staining of mouse heart using 14221-1-AP

IF Staining of Human iPSC derived cardiomyocyte using 14221-1-AP

IF Staining of C2C12 using 14221-1-AP

IF Staining of NIH/3T3 using 14221-1-AP

IF Staining of C2C12 using 14221-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Exoskeleton Partial-Coated Stem Cells for Infarcted Myocardium Restoring

Microskeletal stiffness promotes aortic aneurysm by sustaining pathological vascular smooth muscle cell mechanosensation via Piezo1.

Alkaline nucleoplasm facilitates contractile gene expression in the mammalian heart.

Essential role of MESP1-RING1A complex in cardiac differentiation

Targeting of CAT and VCAM1 as Novel Therapeutic Targets for DMD Cardiomyopathy.

Incomplete Assembly of the Dystrophin-Associated Protein Complex in 2D and 3D-Cultured Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes.

Reviews