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  • Featured Product
  • KD/KO Validated

ACTN4 Monoclonal antibody, PBS Only

ACTN4 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E12B8

Cat no : 66628-1-PBS

Synonyms

ACTININ 4, actinin, alpha 4, ACTN4, Alpha actinin 4, F actin cross linking protein, FSGS, FSGS1, Non muscle alpha actinin 4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ACTN4 Monoclonal antibody (66628-1-Ig)

    WB analysis using 66628-1-Ig (same clone as 66628-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66628-1-Ig (ACTN4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66628-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ACTN4 Monoclonal antibody (66628-1-Ig)

    WB analysis using 66628-1-Ig (same clone as 66628-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66628-1-Ig (ACTN4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66628-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ACTN4 Monoclonal antibody (66628-1-Ig)

    IHC staining of human breast cancer using 66628-1-Ig (same clone as 66628-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66628-1-Ig (ACTN4 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66628-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ACTN4 Monoclonal antibody (66628-1-Ig)

    IHC staining of human breast cancer using 66628-1-Ig (same clone as 66628-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66628-1-Ig (ACTN4 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66628-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using ACTN4 Monoclonal antibody (66628-1-Ig)

    IHC staining of human lung cancer using 66628-1-Ig (same clone as 66628-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66628-1-Ig (ACTN4 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66628-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using ACTN4 Monoclonal antibody (66628-1-Ig)

    IHC staining of human lung cancer using 66628-1-Ig (same clone as 66628-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66628-1-Ig (ACTN4 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66628-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66628-1-PBS targets ACTN4 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ACTN4 fusion protein Ag13549 相同性解析による交差性が予測される生物種
Full Name actinin, alpha 4
Calculated molecular weight 105 kDa
Observed molecular weight 105 and 80 kDa
GenBank accession numberBC005033
Gene symbol ACTN4
Gene ID (NCBI) 81
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ACTN4 (alpha actinin 4) is a ubiquitous actin-binding protein that cross-links actin filaments into bundles to form filopodia, and has a crucial role in cytoskeletal organization. ACTN4 is colocalized with actin stress fibers and is dispersed in the cytoplasm and in the nucleus. Increased levels of ACTN4 have been associated with cancer metastasis, indicating its implication in cancer cell migration and metastasis. Defects in ACTN4 cause glomerular disease. This antibody recognizes 105 and 80 kDa of endogenous ACTN4 protein in various lysates. (22370640, 10700177)