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ACTR3B Polyclonal antibody

ACTR3B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17022-1-AP

Synonyms

Actin-related protein ARP4, Actin-related protein 3B, Actin-like protein 3B, Actin related protein ARP4, Actin related protein 3B

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ACTR3B Polyclonal antibody (17022-1-AP)

    WB analysis using 17022-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17022-1-AP (ACTR3B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using ACTR3B Polyclonal antibody (17022-1-AP)

    WB analysis of A431 using 17022-1-AP

    WB result of ACTR3B antibody (17022-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACTR3B transfected A431 cells.

  • Western Blot (WB) analysis of mouse pancreas tissue using ACTR3B Polyclonal antibody (17022-1-AP)

    WB analysis of mouse pancreas using 17022-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 17022-1-AP (ACTR3B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using ACTR3B Polyclonal antibody (17022-1-AP)

    IHC staining of human stomach cancer using 17022-1-AP

    Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 17022-1-AP (ACTR3B antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using ACTR3B Polyclonal antibody (17022-1-AP)

    IHC staining of human stomach cancer using 17022-1-AP

    Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 17022-1-AP (ACTR3B antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inA431 cells, mouse pancreas tissue, HepG2 cells, K-562 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17022-1-AP targets ACTR3B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACTR3B fusion protein Ag10218 相同性解析による交差性が予測される生物種
Full Name ARP3 actin-related protein 3 homolog B (yeast)
Calculated molecular weight 418 aa, 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC008682
Gene symbol ACTR3B
Gene ID (NCBI) 57180
RRIDAB_2305069
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ACTR3B antibody 17022-1-APDownload protocol
IHC protocol for ACTR3B antibody 17022-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols