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KD/KO Validated

Arp5/ACTR5 Polyclonal antibody

Name: Arp5/ACTR5 Polyclonal antibody
Brand: Proteintech
SKU: 21505-1-AP

Arp5/ACTR5 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 21505-1-AP

Synonyms

Actin related protein 5, ACTR5, Arp5, Arp5/ACTR5, hARP5, Sarcoma antigen NY SAR 16

Validation Data Gallery

Filter:

AllWBIPIF/ICC

WB analysis of mouse cerebellum using 21505-1-AP
mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 21505-1-AP (Arp5/ACTR5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 21505-1-AP
WB result of Arp5/ACTR5 antibody (21505-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Arp5/ACTR5 transfected HEK-293 cells.
WB analysis using 21505-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21505-1-AP (Arp5/ACTR5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 21505-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 21505-1-AP (Arp5/ACTR5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human skeletal muscle using 21505-1-AP
human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21505-1-AP (Arp5/ACTR5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 21505-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 21505-1-AP (Arp5/ACTR5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse ovary using 21505-1-AP
mouse ovary tissue were subjected to SDS PAGE followed by western blot with 21505-1-AP (Arp5/ACTR5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 21505-1-AP
IP result of anti-Arp5/ACTR5 (IP:21505-1-AP, 4ug; Detection:21505-1-AP 1:500) with HEK-293 cells lysate 1120ug.
IF Staining of A549 using 21505-1-AP
Immunofluorescent analysis of A549 cells using 21505-1-AP (Arp5/ACTR5 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of HepG2 using 21505-1-AP
Immunofluorescent analysis of HepG2 cells, using ACTR5 antibody 21505-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Cat No. 21505-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, human skeletal muscle tissue, mouse cerebellum tissue, mouse heart tissue, mouse ovary tissue, rat heart tissue
Positive IP detected in	HEK-293 cells
Positive IF/ICC detected in	A549 cells, HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 4 publications below
IF	See 2 publications below
IP	See 1 publications below

Product Information

21505-1-AP targets Arp5/ACTR5 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, rat, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Arp5/ACTR5 fusion protein Ag15976 相同性解析による交差性が予測される生物種
Full Name	ARP5 actin-related protein 5 homolog (yeast)
Calculated molecular weight	607 aa, 68 kDa
Observed molecular weight	68 kDa
GenBank accession number	BC038402
Gene symbol	ARP5/ACTR5
Gene ID (NCBI)	79913
RRID	AB_10733470
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Arp5 is a member of nuclear actin-related proteins (ARPs) that function as components of the chromatin- remodeling complex. Recently study reported that Arp5 regulates smooth muscle cell differentiation through interaction with myocardin (Myocd). Arp5 has been identified as negative regulator of Myocd, suppressing Myocd activity in the nucleus through tightly binding with Myocd RPEL motifs. Alternative splicing results in two variants of Arp5: a broadly expressed arp5-common, and a smooth muscle restricted arp5-sm. Expression of Arp5-sm protein is strongly suppressed in well-differentiated smooth muscle cells by partial messenger RNA decay and translational suppression. (24567363)

Protocols

Product Specific Protocols
WB protocol for Arp5/ACTR5 antibody 21505-1-AP	Download protocol
IF protocol for Arp5/ACTR5 antibody 21505-1-AP	Download protocol
IP protocol for Arp5/ACTR5 antibody 21505-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	EMBO J LncRNA HAND2-AS1 promotes liver cancer stem cell self-renewal via BMP signaling. Authors - Yanying Wang View Article
rat	WB,IF	J Cell Biol Arp5 is a key regulator of myocardin in smooth muscle cells. Authors - Tsuyoshi Morita KD Validated View Article
mouse	WB,IF,IP	Elife Actin-related protein 5 functions as a novel modulator of MyoD and MyoG in skeletal muscle and in rhabdomyosarcoma. Authors - Tsuyoshi Morita KD Validated View Article
human	WB	Cell Rep Activation of the CARD8 Inflammasome Requires a Disordered Region. Authors - Ashley J Chui View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Arp5/ACTR5 Polyclonal antibody

Arp5/ACTR5 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of mouse cerebellum using 21505-1-AP

WB analysis of HEK-293 using 21505-1-AP

WB analysis using 21505-1-AP

WB analysis of mouse heart using 21505-1-AP

WB analysis of human skeletal muscle using 21505-1-AP

WB analysis of HEK-293 using 21505-1-AP

WB analysis of mouse ovary using 21505-1-AP

IP experiment of HEK-293 using 21505-1-AP

IF Staining of A549 using 21505-1-AP

IF Staining of HepG2 using 21505-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

LncRNA HAND2-AS1 promotes liver cancer stem cell self-renewal via BMP signaling.

Arp5 is a key regulator of myocardin in smooth muscle cells.

Actin-related protein 5 functions as a novel modulator of MyoD and MyoG in skeletal muscle and in rhabdomyosarcoma.

Activation of the CARD8 Inflammasome Requires a Disordered Region.