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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ACVR1B Recombinant antibody (83026-6-RR)

    WB analysis using 83026-6-RR (same clone as 83026-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83026-6-RR (ACVR1B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83026-6-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using ACVR1B Recombinant antibody (83026-6-RR)

    IHC staining of rat brain using 83026-6-RR (same clone as 83026-6-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 83026-6-RR (ACVR1B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83026-6-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ACVR1B Recombinant antibody (83026-6-RR)

    IHC staining of mouse brain using 83026-6-RR (same clone as 83026-6-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83026-6-RR (ACVR1B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83026-6-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A549 cells using ACVR1B Recombinant antibody (83026-6-RR)

    FC experiment of A549 using 83026-6-RR (same clone as 83026-6-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.4 ug ACVR1B Recombinant antibody (83026-6-RR, Clone:230260C1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83026-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83026-6-RR

    Affinity and Kinetic Characterization of 83026-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83026-6-RR against Human ACVR1B were performed. The affinity constant is 35.6 pM.

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ACVR1B Recombinant antibody, PBS Only

ACVR1B Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 83026-6-PBS
Clone No.230260C1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), Indirect ELISA

Synonyms

Activin receptor-like kinase 4, Activin receptor type-1B, Activin receptor type 1B, Activin receptor like kinase 4, activin A receptor, type IB

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83026-6-PBS targets ACVR1B in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ACVR1B fusion protein Ag0129 相同性解析による交差性が予測される生物種
Full Name activin A receptor, type IB
Calculated molecular weight 57 kDa
Observed molecular weight52 kDa
GenBank accession numberBC000254
Gene Symbol ACVR1B
Gene ID (NCBI) 91
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP36896
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ACVR1B (ALK4, activin A receptor type 1B) is a transforming growth factor-beta (TGF-beta) superfamily member (PMID: 37020544). A potential role for ACVR1B in regulating muscle mass is that it is part of the transforming growth factor b (TGFb) pathway regulating myostatin (PMID: 21063444).