ACVRL1 Polyclonal antibody
ACVRL1 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
Cat no : 14745-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | human placenta tissue |
Positive IP detected in | Jurkat cells |
Positive IHC detected in | human heart tissue, human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 6 publications below |
IHC | See 2 publications below |
IP | See 1 publications below |
FC | See 1 publications below |
Product Information
14745-1-AP targets ACVRL1 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | ACVRL1 fusion protein Ag6449 相同性解析による交差性が予測される生物種 |
Full Name | activin A receptor type II-like 1 |
Calculated molecular weight | 56 kDa |
Observed molecular weight | 63 kDa |
GenBank accession number | BC042637 |
Gene symbol | ACVRL1 |
Gene ID (NCBI) | 94 |
RRID | AB_2222474 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ACVRL1 (also known as ALK1) is a type I cell-surface receptor for the TGF-beta superfamily of ligands. It shares with other type I receptors a high degree of similarity in serine-threonine kinase subdomains, a glycine- and serine-rich region (called the GS domain) preceding the kinase domain, and a short C-terminal tail. ACVRL1 is highly expressed in endothelial cells and has a critical role in the control of blood vessel development and repair (PMID: 8640225). Mutations in the ACVRL1 gene are associated with hemorrhagic telangiectasia type 2.
Protocols
Product Specific Protocols | |
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WB protocol for ACVRL1 antibody 14745-1-AP | Download protocol |
IHC protocol for ACVRL1 antibody 14745-1-AP | Download protocol |
IP protocol for ACVRL1 antibody 14745-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Mol Cell OTULIN allies with LUBAC to govern angiogenesis by editing ALK1 linear polyubiquitin. | ||
Nat Commun Clonal hematopoiesis with JAK2V617F promotes pulmonary hypertension with ALK1 upregulation in lung neutrophils. | ||
J Mol Cell Cardiol BMP9 attenuates occurrence of venous malformation by maintaining endothelial quiescence and strengthening vessel walls via SMAD1/5/ID1/α-SMA pathway. | ||
Cell Biol Int CHRDL2 promotes osteosarcoma cell proliferation and metastasis through the BMP-9/ PI3K/ AKT pathway. | ||
J Thorac Dis Pulmonary inflammatory myofibroblastic tumor versus IgG4-related inflammatory pseudotumor: differential diagnosis based on a case series. | ||
Biosci Biotechnol Biochem BMP9 functions as a negative regulator in the myogenic differentiation of primary mouse myoblasts |