ACVRL1 Polyclonal antibody

ACVRL1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14745-1-AP

Synonyms

Activin receptor like kinase 1, ACVRL1, ACVRLK1, ALK 1, ALK1, HHT, HHT2, ORW2, SKR3, TSR I


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Tested Applications

Positive WB detected inhuman placenta tissue
Positive IP detected inJurkat cells
Positive IHC detected inhuman heart tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14745-1-AP targets ACVRL1 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACVRL1 fusion protein Ag6449 相同性解析による交差性が予測される生物種
Full Name activin A receptor type II-like 1
Calculated molecular weight 56 kDa
Observed molecular weight 63 kDa
GenBank accession numberBC042637
Gene symbol ACVRL1
Gene ID (NCBI) 94
RRIDAB_2222474
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ACVRL1 (also known as ALK1) is a type I cell-surface receptor for the TGF-beta superfamily of ligands. It shares with other type I receptors a high degree of similarity in serine-threonine kinase subdomains, a glycine- and serine-rich region (called the GS domain) preceding the kinase domain, and a short C-terminal tail. ACVRL1 is highly expressed in endothelial cells and has a critical role in the control of blood vessel development and repair (PMID: 8640225). Mutations in the ACVRL1 gene are associated with hemorrhagic telangiectasia type 2.

Protocols

Product Specific Protocols
WB protocol for ACVRL1 antibody 14745-1-APDownload protocol
IHC protocol for ACVRL1 antibody 14745-1-APDownload protocol
IP protocol for ACVRL1 antibody 14745-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,humanWB

Mol Cell

OTULIN allies with LUBAC to govern angiogenesis by editing ALK1 linear polyubiquitin.

Authors - Yesheng Fu
humanWB,FC

Nat Commun

Clonal hematopoiesis with JAK2V617F promotes pulmonary hypertension with ALK1 upregulation in lung neutrophils.

Authors - Yusuke Kimishima
humanWB

J Mol Cell Cardiol

BMP9 attenuates occurrence of venous malformation by maintaining endothelial quiescence and strengthening vessel walls via SMAD1/5/ID1/α-SMA pathway.

Authors - Yongyun Li
humanWB,IP

Cell Biol Int

CHRDL2 promotes osteosarcoma cell proliferation and metastasis through the BMP-9/ PI3K/ AKT pathway.

Authors - Houping Chen
humanIHC

J Thorac Dis

Pulmonary inflammatory myofibroblastic tumor versus IgG4-related inflammatory pseudotumor: differential diagnosis based on a case series.

Authors - Longfei Zhu
mouseWB,IHC

Biosci Biotechnol Biochem

BMP9 functions as a negative regulator in the myogenic differentiation of primary mouse myoblasts

Authors - Xiya Lu