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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ACVRL1 Recombinant antibody, PBS Only (Capture/Detector)

ACVRL1 Recombinant Antibody for IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241686B9

Cat no : 84421-1-PBS

Synonyms

ALK1, ALK 1, ACVRLK1, Activin receptor-like kinase 1, Activin receptor like kinase 1

Validation Data Gallery

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  • Sandwich ELISA standard curve of MP01306-2

    Sandwich ELISA standard curve of MP01306-2

    Sandwich ELISA standard curve of MP01306-2, Human ACVRL1 Recombinant Matched Antibody Pair - PBS only. 84421-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2132. 84421-2-PBS was HRP conjugated as the detection antibody. Range: 0.781-50 ng/mL.

  • Sample test of MP01306-2

    Sample test of MP01306-2

    The mean ACVRL1 concentration was determined to be 34.48 ng/mL in HUVEC cell extract based on a 0.91 mg/mL extract load, 1108.74 ng/mL in HL-60 cell extract based on a 3.00 mg/mL extract load and 176.59 ng/mL in U-937 cell extract based on a 1.40 mg/mL extract load.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using ACVRL1 Recombinant antibody (84421-1-RR)

    IF Staining of Jurkat using 84421-1-RR (same clone as 84421-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ACVRL1 antibody (84421-1-RR, Clone: 241686B9 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84421-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01306-1

    Cytometric bead array standard curve of MP01306-1

    Cytometric bead array standard curve of MP01306-1, ACVRL1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84421-2-PBS. Detection antibody: 84421-1-PBS. Standard: Eg2132. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 84421-1-RR

    Affinity and Kinetic Characterization of 84421-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84421-1-RR against Human ACVRL1 were performed. The affinity constant is 0.727 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84421-1-PBS targets ACVRL1 as part of a matched antibody pair:

MP01306-1: 84421-2-PBS capture and 84421-1-PBS detection (validated in Cytometric bead array)

MP01306-2: 84421-1-PBS capture and 84421-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FusionProtein 相同性解析による交差性が予測される生物種
Full Name activin A receptor type II-like 1
Calculated molecular weight 56 kDa
GenBank accession numberBC042637
Gene symbol ACVRL1
Gene ID (NCBI) 94
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ACVRL1 (also known as ALK1) is a type I cell-surface receptor for the TGF-beta superfamily of ligands. It shares with other type I receptors a high degree of similarity in serine-threonine kinase subdomains, a glycine- and serine-rich region (called the GS domain) preceding the kinase domain, and a short C-terminal tail. ACVRL1 is highly expressed in endothelial cells and has a critical role in the control of blood vessel development and repair (PMID: 8640225). Mutations in the ACVRL1 gene are associated with hemorrhagic telangiectasia type 2.