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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Aminoacylase 1 Polyclonal antibody

Aminoacylase 1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11316-1-AP

Synonyms

ACY 1, ACY1, ACY1D, ACYLASE, Aminoacylase 1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using Aminoacylase 1 Polyclonal antibody (11316-1-AP)

    WB analysis using 11316-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11316-1-AP (Aminoacylase 1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Aminoacylase 1 Polyclonal antibody (11316-1-AP)

    IHC staining of human prostate cancer using 11316-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11316-1-AP (Aminoacylase 1 antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Aminoacylase 1 Polyclonal antibody (11316-1-AP)

    IHC staining of human prostate cancer using 11316-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11316-1-AP (Aminoacylase 1 antibody at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inK-562 cells, PC-3 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

11316-1-AP targets Aminoacylase 1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Aminoacylase 1 fusion protein Ag1813 相同性解析による交差性が予測される生物種
Full Name aminoacylase 1
Calculated molecular weight40.8 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC014112
Gene symbol ACY1
Gene ID (NCBI) 95
RRIDAB_2877757
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Aminoacylase 1 antibody 11316-1-APDownload protocol
IHC protocol for Aminoacylase 1 antibody 11316-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Heliyon

FYN-mediated phosphorylation of BCKDK at Y151 promotes GBM proliferation by increasing the oncogenic metabolite N-acetyl-L-alanine

Authors - Ling Zou
View Article