ADA Polyclonal antibody
ADA Polyclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
Cat no : 13328-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, Jurkat cells, Sp2/0 cells |
Positive IHC detected in | human stomach tissue, human cervical cancer tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse small intestine tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 7 publications below |
IHC | See 2 publications below |
Product Information
13328-1-AP targets ADA in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | ADA fusion protein Ag4128 相同性解析による交差性が予測される生物種 |
Full Name | adenosine deaminase |
Calculated molecular weight | 363 aa, 41 kDa |
Observed molecular weight | 45-47 kDa |
GenBank accession number | BC040226 |
Gene symbol | ADA |
Gene ID (NCBI) | 100 |
RRID | AB_2877939 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
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WB protocol for ADA antibody 13328-1-AP | Download protocol |
IHC protocol for ADA antibody 13328-1-AP | Download protocol |
IF protocol for ADA antibody 13328-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Front Cell Dev Biol Thyroid Hormone-Regulated Expression of Period2 Promotes Liver Urate Production. | ||
Eur J Pharmacol Adenosine kinase (ADK) inhibition with ABT-702 induces ADK protein degradation and a distinct form of sustained cardioprotection. | ||
Cell Death Dis Inosine enhances tumor mitochondrial respiration by inducing Rag GTPases and nascent protein synthesis under nutrient starvation | ||
bioRxiv The SAGA acetyltransferase module is required for the maintenance of MAF and MYC oncogenic gene expression programs in multiple myeloma | ||
NPJ Biofilms Microbiomes Lactobacillus rhamnosus GG ameliorates hyperuricemia in a novel model | ||
Animal Model Exp Med The cumulative analgesic effect of repeated electroacupuncture is modulated by Adora3 in the SCDH of mice with neuropathic pain |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Neta (Verified Customer) (07-28-2022) | antibody worked, signal slightly different than reported but overall OK.
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FH Ryan (Verified Customer) (02-28-2019) | Tissue was fixed in PFA with NaCit antigen retrieval ph=6.
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