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ADAM10 Polyclonal antibody

ADAM10 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25900-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Mammalian disintegrin-metalloprotease, MADM, KUZ, EC:3.4.24.81, Disintegrin and metalloproteinase domain-containing protein 10

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inNIH/3T3 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman lung cancer tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25900-1-AP targets ADAM10 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADAM10 fusion protein Ag23078 相同性解析による交差性が予測される生物種
Full Name ADAM metallopeptidase domain 10
Calculated molecular weight 748 aa, 84 kDa
Observed molecular weight 60-70 kDa, 90 kDa
GenBank accession numberBC126253
Gene Symbol ADAM10
Gene ID (NCBI) 102
RRIDAB_2880291
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14672
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ADAM10, a member of the ADAM (a disintegrin and metalloprotease) family, is widely expressed in the brain, the spinal cord, and the visual system during development.MW of premature ADAM10 is 90 kDa and mature ADAM10 is 60-70 kDa (PMID: 24404179).

Protocols

Product Specific Protocols
WB protocol for ADAM10 antibody 25900-1-APDownload protocol
IHC protocol for ADAM10 antibody 25900-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Aging Cell

Microglia-derived Galectin-9 drives amyloid-β pathology in Alzheimer's disease

Authors - Guoxin Zhang
humanWB,IF

Cell Death Dis

Cytoplasmic fragment of CD147 generated by regulated intramembrane proteolysis contributes to HCC by promoting autophagy.

Authors - Bo Wu
human,mouseWB,IHC,IF

Pharmacol Res

IDO1 can impair NK cells function against non-small cell lung cancer by downregulation of NKG2D Ligand via ADAM10.

Authors - Xin Fang
humanWB

Cell Prolif

Increased SPON1 promotes pancreatic ductal adenocarcinoma progression by enhancing IL-6 trans-signalling.

Authors - Yanmiao Huo
mouseWB

J Neurosci

Novel Social Stimulation Ameliorates Memory Deficit in Alzheimer's Disease Model through Activating α-Secretase

Authors - Qiaoyun Ren
humanWB

Front Cell Dev Biol

NLGN3 Upregulates Expression of ADAM10 to Promote the Cleavage of NLGN3 via Activating the LYN Pathway in Human Gliomas.

Authors - Ning-Ning Dang
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