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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ADAM12 Polyclonal antibody

ADAM12 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26928-1-AP

Synonyms

ADAM 12, ADAM12, MCMP, MCMPMltna, Meltrin alpha, MLTN, MLTNA

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ADAM12 Polyclonal antibody (26928-1-AP)

    WB analysis using 26928-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26928-1-AP (ADAM12 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using ADAM12 Polyclonal antibody (26928-1-AP)

    WB analysis of A431 using 26928-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 26928-1-AP (ADAM12 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using ADAM12 Polyclonal antibody (26928-1-AP)

    WB analysis of mouse brain using 26928-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 26928-1-AP (ADAM12 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using ADAM12 Polyclonal antibody (26928-1-AP)

    WB analysis of A549 using 26928-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 26928-1-AP (ADAM12 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using ADAM12 Polyclonal antibody (26928-1-AP)

    IHC staining of human endometrial cancer using 26928-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 26928-1-AP (ADAM12 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using ADAM12 Polyclonal antibody (26928-1-AP)

    IHC staining of human endometrial cancer using 26928-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 26928-1-AP (ADAM12 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse brain tissue, A431 cells, A549 cells, rat brain tissue
Positive IHC detected inhuman endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26928-1-AP is ADAM12 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADAM12 fusion protein Ag17474 相同性解析による交差性が予測される生物種
Full Name ADAM metallopeptidase domain 12
Calculated molecular weight 909 aa, 100 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC060804
Gene symbol ADAM12
Gene ID (NCBI) 8038
RRIDAB_2880689
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ADAM12, also named as MLTN and Meltrin-alpha, is involved in skeletal muscle regeneration, specifically at the onset of cell fusion. It is also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors. ADAM12 is expressed in human malignant tumors. It is involved in the regulation of growth factor activities and integrin functions, leading to promotion of cell growth and invasion, although the precise mechanisms of these are not clear at the present time.(PMID:17355265) ADAM12's ability to degrade extracellular matrix components likely allows it to detach cancer cells from the basement membrane and assist them on their route to metastasis. But the protein's role not just as a biomarker of breast cancer but as a gateway to cancer cell migration is only now being understood. ADAM12 has 2 isoforms with the calculated molecular mass of 100 and 80 kDa, and this antibody can recognize all isoforms of ADAM12. ADAM12 has 4 forms: 120kd full-length form, 90 kDa mature (processed form that lack the prodomain), 50-68 kDa degradation product and 27 kDa prodomain.

Protocols

Product Specific Protocols
WB protocol for ADAM12 antibody 26928-1-APDownload protocol
IHC protocol for ADAM12 antibody 26928-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols