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  • Featured Product
  • KD/KO Validated

ADAM28 Polyclonal antibody, PBS Only

ADAM28 Polyclonal Antibody for WB, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 22234-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse lung tissue using ADAM28 Polyclonal antibody (22234-1-AP)

    WB analysis of mouse lung using 22234-1-AP (same clone as 22234-1-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 22234-1-AP (ADAM28 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22234-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse spleen tissue using ADAM28 Polyclonal antibody (22234-1-AP)

    WB analysis of mouse spleen using 22234-1-AP (same clone as 22234-1-PBS)

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 22234-1-AP (ADAM28 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22234-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using ADAM28 Polyclonal antibody (22234-1-AP)

    WB analysis of mouse liver using 22234-1-AP (same clone as 22234-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 22234-1-AP (ADAM28 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22234-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of BxPC-3 cells using ADAM28 Polyclonal antibody (22234-1-AP)

    WB analysis of BxPC-3 using 22234-1-AP (same clone as 22234-1-PBS)

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 22234-1-AP (ADAM28 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22234-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse lung tissue using ADAM28 Polyclonal antibody (22234-1-AP)

    WB analysis of mouse lung using 22234-1-AP (same clone as 22234-1-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 22234-1-AP (ADAM28 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22234-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-13 cells using ADAM28 Polyclonal antibody (22234-1-AP)

    WB analysis of PC-13 using 22234-1-AP (same clone as 22234-1-PBS)

    PC-13 cells were subjected to SDS PAGE followed by western blot with 22234-1-AP (ADAM28 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22234-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse small intestine tissue using ADAM28 Polyclonal antibody (22234-1-AP)

    WB analysis of mouse small intestine using 22234-1-AP (same clone as 22234-1-PBS)

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 22234-1-AP (ADAM28 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22234-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse colon tissue using ADAM28 Polyclonal antibody (22234-1-AP)

    WB analysis of mouse colon using 22234-1-AP (same clone as 22234-1-PBS)

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 22234-1-AP (ADAM28 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22234-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse lung tissue using ADAM28 Polyclonal antibody (22234-1-AP)

    IP experiment of mouse lung using 22234-1-AP (same clone as 22234-1-PBS)

    IP result of anti-ADAM28 (IP:22234-1-AP, 4ug; Detection:22234-1-AP 1:800) with mouse lung tissue lysate 3600ug. This data was developed using the same antibody clone with 22234-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22234-1-PBS targets ADAM28 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADAM28 fusion protein Ag17586 相同性解析による交差性が予測される生物種
Full Name ADAM metallopeptidase domain 28
Calculated molecular weight 775 aa, 87 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC136478
Gene symbol ADAM28
Gene ID (NCBI) 10863
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ADAM28 belongs to a family of cell surface and secreted glycoproteins that possess both proteolytic and adhesive properties. ADAM28 is predominantly expressed by carcinoma cells in the breast carcinoma tissues as protein bands of 42 kDa and 55/57 kDa, which correspond to the active forms of ADAM28s and ADAM28m, respectively.In human non-small cell lung carcinomas and breast carcinomas, ADAM28 is overexpressed predominantly by carcinoma cells, and the expression correlates with carcinoma cell proliferation and lymph node metastasis(PMID:19601836).