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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using ADAM5 Polyclonal antibody (22147-1-AP)

    WB analysis of HeLa using 22147-1-AP (same clone as 22147-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 22147-1-AP (ADAM5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22147-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using ADAM5 Polyclonal antibody (22147-1-AP)

    WB analysis of MCF-7 using 22147-1-AP (same clone as 22147-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 22147-1-AP (ADAM5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22147-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using ADAM5 Polyclonal antibody (22147-1-AP)

    IHC staining of human testis using 22147-1-AP (same clone as 22147-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 22147-1-AP (ADAM5 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22147-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using ADAM5 Polyclonal antibody (22147-1-AP)

    IHC staining of human testis using 22147-1-AP (same clone as 22147-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 22147-1-AP (ADAM5 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22147-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ADAM5 Polyclonal antibody (22147-1-AP)

    IF Staining of HeLa using 22147-1-AP (same clone as 22147-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ADAM5 antibody (22147-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 22147-1-PBS in a different storage buffer formulation.

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ADAM5 Polyclonal antibody, PBS Only

ADAM5 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 22147-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

ADAM5P, TMDCII

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22147-1-PBS targets ADAM5 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADAM5 fusion protein Ag17590 相同性解析による交差性が予測される生物種
Full Name ADAM metallopeptidase domain 5 pseudogene
Calculated molecular weight 412 aa, 47 kDa
Observed molecular weight 36-50 kDa
GenBank accession numberBC067864
Gene Symbol ADAM5/ADAM5P
Gene ID (NCBI) 255926
RRIDAB_11182171
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

ADAM5(putative disintegrin and metalloproteinase domain-containing protein 5) is also named as ADAM5P, TMDC2(tMDCII). The tMDC II, abundantly expressed, sperm surface MDC proteins identified in rodent and macaque systems, is also nonfunctional in the human(PMID:10417343). It has 7 isoforms produced by alternative splicing. It is expressed in testis highly by northern blot. This antibody is specific to ADAM5.