Validation Data Gallery
Tested Applications
| Positive WB detected in | BxPC-3 cells, mouse pancreas tissue |
| Positive IHC detected in | human tonsillitis tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 8 publications below |
| IHC | See 6 publications below |
| IP | See 1 publications below |
Product Information
23778-1-AP targets ADAM8 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ADAM8 fusion protein Ag17262 相同性解析による交差性が予測される生物種 |
| Full Name | ADAM metallopeptidase domain 8 |
| Calculated molecular weight | 824 aa, 89 kDa |
| Observed molecular weight | 65 kDa |
| GenBank accession number | BC115404 |
| Gene Symbol | ADAM8 |
| Gene ID (NCBI) | 101 |
| RRID | AB_2879321 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P78325 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ADAMs are a large family of transmembrane proteins that are involved in proteolysis, making them candidates for mediating the remodeling of the extracellular matrix(ECM). ADAM8, one of the members of the ADAM family, is overexpressed in various human tumors. It has been shown previously that hypoxia influences the expression of some ADAM proteins. The protein found to be expressed in two active forms(90 kDa and 60 kDa): potential shedding protein and remnant protein in the pancreatic tissues under chronic pancreatitis and pancreatic cancer(PMID:18566576). The full length protein has a signal peptide and four glycosylation sites. This antibody is specific to ADAM8.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ADAM8 antibody 23778-1-AP | Download protocol |
| IHC protocol for ADAM8 antibody 23778-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Neurosci Matrix metalloprotease-mediated cleavage of neural glial-related cell adhesion molecules activates quiescent olfactory stem cells via EGFR. | ||
Exp Anim Suppression of ADAM8 attenuates angiotensin II-induced cardiac fibrosis and endothelial-mesenchymal transition via inhibiting TGF-β1/Smad2/Smad3 pathways.
| ||
J Healthc Eng ADAM8 Activates NLRP3 Inflammasome to Promote Cerebral Ischemia-Reperfusion Injury. | ||
Pathol Oncol Res High ADAM8 expression is associated with poor prognosis in patients with hepatocellular carcinoma. | ||
Int J Mol Med ADAM17 promotes lymph node metastasis in gastric cancer via activation of the Notch and Wnt signaling pathways. | ||
Nat Med Translation control of the immune checkpoint in cancer and its therapeutic targeting. |