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Validation Data Gallery

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  • Western Blot (WB) analysis of human testis tissue using ADAM8 Monoclonal antibody (68113-1-Ig)

    WB analysis of human testis using 68113-1-Ig (same clone as 68113-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 68113-1-Ig (ADAM8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68113-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HaCaT cells using ADAM8 Monoclonal antibody (68113-1-Ig)

    WB analysis of HaCaT using 68113-1-Ig (same clone as 68113-1-PBS)

    HaCaT cells were subjected to SDS PAGE followed by western blot with 68113-1-Ig (ADAM8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68113-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using ADAM8 Monoclonal antibody (68113-1-Ig)

    WB analysis of A431 using 68113-1-Ig (same clone as 68113-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 68113-1-Ig (ADAM8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68113-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ADAM8 Monoclonal antibody (68113-1-Ig)

    WB analysis using 68113-1-Ig (same clone as 68113-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68113-1-Ig (ADAM8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68113-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A431 cells using ADAM8 Monoclonal antibody (68113-1-Ig)

    FC experiment of A431 using 68113-1-Ig (same clone as 68113-1-PBS)

    1x10^6 A431 cells were surface stained with 0.8 ug ADAM8 Monoclonal antibody (68113-1-Ig, Clone:3A12H2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were not fixed. This data was developed using the same antibody clone with 68113-1-PBS in a different storage buffer formulation.

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ADAM8 Monoclonal antibody, PBS Only

ADAM8 Monoclonal Antibody for WB, FC, Indirect ELISA
Cat No. 68113-1-PBS
Clone No.3A12H2

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, FC, Indirect ELISA

Synonyms

MS2, EC:3.4.24.-, Disintegrin and metalloproteinase domain-containing protein 8, Cell surface antigen MS2, CD156a

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68113-1-PBS targets ADAM8 in WB, FC, Indirect ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ADAM8 fusion protein Ag17262 相同性解析による交差性が予測される生物種
Full Name ADAM metallopeptidase domain 8
Calculated molecular weight 824 aa, 89 kDa
Observed molecular weight90 kDa
GenBank accession numberBC115404
Gene Symbol ADAM8
Gene ID (NCBI) 101
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP78325
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

ADAM8 is a type I transmembrane (TM) glycoprotein consisting of an N-terminal prodomain, followed by a metalloproteinase-, disintegrin (DIS)-, cysteine-rich-, epidermal growth factor (EGF)-like- and TM domain and a cytoplasmic tail. Expression levels of ADAM8 in normal tissue are typically low and limited to a few distinct cell types in the lymphatic organs as components of the immune system, the central nervous system, bone, and the lung. This antibody can be used to detect endogenous ADAM8 with an apparent molecular weight of 90 kDa (the calculated MW ). ADAM8 also can be detectable as a band with a molecular weight (MW) of 98 kDa, slightly larger than the calculated MW (90 kDa) (PMID:11050116).