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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ADAP2 Polyclonal antibody

ADAP2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13706-1-AP

Synonyms

ADAP2, ArfGAP with dual PH domains 2, cent b, CENTA2, Centaurin alpha 2, Cnt a2, HSA272195

Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using ADAP2 Polyclonal antibody (13706-1-AP)

    WB analysis of human placenta using 13706-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 13706-1-AP (ADAP2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using ADAP2 Polyclonal antibody (13706-1-AP)

    WB analysis of mouse liver using 13706-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 13706-1-AP (ADAP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using ADAP2 Polyclonal antibody (13706-1-AP)

    IHC staining of mouse spleen using 13706-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 13706-1-AP (ADAP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using ADAP2 Polyclonal antibody (13706-1-AP)

    IHC staining of mouse spleen using 13706-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 13706-1-AP (ADAP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inhuman placenta tissue, mouse liver tissue
Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13706-1-AP targets ADAP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADAP2 fusion protein Ag4641 相同性解析による交差性が予測される生物種
Full Name ArfGAP with dual PH domains 2
Calculated molecular weight 380 aa, 44 kDa
Observed molecular weight 44 kDa
GenBank accession numberBC033758
Gene symbol ADAP2
Gene ID (NCBI) 55803
RRIDAB_10644119
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ADAP2 antibody 13706-1-APDownload protocol
IHC protocol for ADAP2 antibody 13706-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols