ADAT2 Polyclonal antibody

ADAT2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 13621-1-AP

Synonyms

tRNA-specific adenosine deaminase 2, TAD2, EC:3.5.4.33, Deaminase domain-containing protein 1, DEADC1


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Tested Applications

Positive WB detected inA431 cells, mouse brain tissue, mouse pancreas tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman hepatocirrhosis tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

13621-1-AP targets ADAT2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADAT2 fusion protein Ag4529 相同性解析による交差性が予測される生物種
Full Name adenosine deaminase, tRNA-specific 2, TAD2 homolog (S. cerevisiae)
Calculated molecular weight 191 aa, 21 kDa
Observed molecular weight 25 kDa
GenBank accession numberBC037955
Gene symbol ADAT2
Gene ID (NCBI) 134637
RRIDAB_10642142
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ADAT2(tRNA-specific adenosine deaminase 2) is also named as DEADC1 and Belongs to the cytidine and deoxycytidylate deaminase family. It is a 24-kDa molecular mass protein that harbors all the conserved motifs required for deamination. ADAT2 and ADAT3 can function as a heterodimer which catalyses inosine formation at the wobble position (position 34) in eukaryotic tRNAs(PMID:12457566). It has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for ADAT2 antibody 13621-1-APDownload protocol
IHC protocol for ADAT2 antibody 13621-1-APDownload protocol
IF protocol for ADAT2 antibody 13621-1-APDownload protocol
IP protocol for ADAT2 antibody 13621-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nucleic Acids Res

The structure of the mouse ADAT2/ADAT3 complex reveals the molecular basis for mammalian tRNA wobble adenosine-to-inosine deamination.

Authors - Elizabeth Ramos-Morales