Validation Data Gallery
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Tested Applications
| Positive IHC detected in | rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 1 publications below |
Product Information
30153-1-AP targets ADCY5 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ADCY5 fusion protein Ag32812 相同性解析による交差性が予測される生物種 |
| Full Name | adenylate cyclase 5 |
| Calculated molecular weight | 139 kDa |
| GenBank accession number | NM_183357 |
| Gene Symbol | ADCY5 |
| Gene ID (NCBI) | 111 |
| RRID | AB_3086248 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95622 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ADCY5 belongs to the adenylate cyclase (ADCY) superfamily which is a group of glycoproteins regulating intracellular signaling (PMID: 35832555). ADCY5 is expressed only in neurons and all neurons express ADCY5 (PMID: 31970214). Gain-of-function mutations in ADCY5 is linked to a rare movement-onset disorder called ADCY5-related dyskinesia (PMID: 32627162).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ADCY5 antibody 30153-1-AP | Download protocol |
| IF protocol for ADCY5 antibody 30153-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Pharmacol Comprehensively prognostic and immunological analyses of GLP-1 signaling-related genes in pan-cancer and validation in colorectal cancer |