ADH1B Polyclonal antibody
ADH1B Polyclonal Antibody for WB, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
mouse, rat
Applications
WB, IP, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 17165-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse liver tissue |
Positive IP detected in | mouse liver tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 4 publications below |
IHC | See 1 publications below |
IF | See 2 publications below |
Product Information
17165-1-AP targets ADH1B in WB, IP, IF, IHC, ELISA applications and shows reactivity with mouse, rat samples.
Tested Reactivity | mouse, rat |
Cited Reactivity | rat, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | ADH1B fusion protein Ag10630 相同性解析による交差性が予測される生物種 |
Full Name | alcohol dehydrogenase 1B (class I), beta polypeptide |
Calculated molecular weight | 375 aa, 40 kDa |
Observed molecular weight | 40 kDa |
GenBank accession number | BC033009 |
Gene symbol | ADH1B |
Gene ID (NCBI) | 125 |
RRID | AB_2878353 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The ADH1B gene encodes the beta subunit of class I alcohol dehydrogenase (ADH), an enzyme that catalyzes the rate-limiting step for ethanol metabolism: the oxidation of alcohol to acetaldehyde. Class 1 ADH is a homo- or heterodimeric molecule, formed by the association of 3 types of class I ADH subunits, alpha (ADH1A), beta, and gamma(ADH1C). ADH1B is also named as ADH2 and belongs to the zinc-containing alcohol dehydrogenase family. This protein can exsit as a homodimer(PMID:19365573).
Protocols
Product Specific Protocols | |
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WB protocol for ADH1B antibody 17165-1-AP | Download protocol |
IP protocol for ADH1B antibody 17165-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Death Discov Senescence as a dictator of patient outcomes and therapeutic efficacies in human gastric cancer. | ||
Endocrinology De novo-synthesized retinoic acid in ovarian antral follicles enhances FSH-mediated ovarian follicular cell differentiation and female fertility. | ||
Arch Biochem Biophys Baicalin ameliorates alcohol-induced hepatic steatosis by suppressing SREBP1c elicited PNPLA3 competitive binding to ATGL. | ||
Exp Eye Res Proteomic analysis reveals the heterogeneity of metabolic reprogramming in lacrimal gland tumors. | ||
J Sci Food Agric Probiotic-fermented Pueraria lobata (Willd.) Ohwi alleviates alcoholic liver injury by enhancing antioxidant defense and modulating gut microbiota | ||
Front Pharmacol Identification of afatinib-associated ADH1B and potential small-molecule drugs targeting ADH1B for hepatocellular carcinoma |